What Is Polysorbate‑80 (Tween‑80) and Its Scientific Applications

Polysorbate‑80, also known as Tween‑80, is a widely used non-ionic surfactant and emulsifier in laboratory and industrial applications. Its solubilizing properties, biocompatibility, and versatility make it an essential reagent for researchers. This article introduces its chemical properties, common applications, and benefits in scientific research.

Chemical Properties of Polysorbate‑80

Polysorbate‑80 (CAS 9005‑65‑6) is a polyoxyethylene sorbitan monooleate with an amphiphilic structure, allowing it to reduce surface tension between oil and water phases. This property is critical for creating stable emulsions and solubilizing hydrophobic compounds.

• 💧 Appearance: Viscous, pale yellow liquid
• 🔬 Solubility: Soluble in water and various organic solvents
• ⚙️ Functionality: Emulsifier, solubilizer, dispersing agent
• 🛡️ Stability: Stable across a wide range of temperatures and pH values

For detailed chemical specifications, visit the Biofargo Polysorbate‑80 product page.

Applications in Laboratory Research

Protein Solubilization and Enzyme Assays

Polysorbate‑80 helps to solubilize hydrophobic proteins or compounds, preventing aggregation and ensuring uniform dispersion in solutions. It is commonly added to enzyme assays and buffer solutions to improve reaction efficiency.

Emulsification in Experimental Formulations

Its non-ionic emulsifying property allows researchers to stabilize oil-in-water mixtures, which is critical for micelle formation, reagent formulation, and consistent experimental results.

Cell Culture and Biocompatibility

Polysorbate‑80 exhibits low cytotoxicity, making it suitable for inclusion in cell culture media to improve solubility of hydrophobic molecules without harming cells.

Advantages of Using Biofargo Polysorbate‑80

Learn more and purchase at the Biofargo Polysorbate‑80 product page.