Using Polysorbate‑80 to Improve Solubility and Dispersion in the Laboratory

Tween‑80 (Polysorbate‑80) is a widely used non-ionic surfactant that enhances the solubility and dispersion of hydrophobic compounds in laboratory solutions. This article provides a practical guide on how to effectively use Tween‑80 in experiments to improve solution homogeneity and prevent aggregation.

Why Use Tween‑80 for Solubility and Dispersion

Hydrophobic proteins, enzymes, or compounds tend to aggregate in aqueous solutions, which can affect experimental outcomes. Tween‑80 acts as a solubilizer by:

• 💧 Reducing surface tension: Helps dissolve hydrophobic molecules uniformly.
• ⚙️ Forming micelles: Encapsulates hydrophobic compounds for better dispersion.
• 🔬 Maintaining stability: Prevents aggregation and precipitation in buffers and media.

Step-by-Step Laboratory Usage Guide

1. Determine the required concentration of Tween‑80 based on the compound’s hydrophobicity (usually 0.01–0.1% w/v).
2. Add Tween‑80 gradually to the aqueous solution while stirring to avoid foam formation.
3. Check for complete dissolution and uniform dispersion visually or by gentle vortexing.
4. Use immediately in experiments or store under recommended conditions to maintain stability.

Following these steps ensures consistent solubility and prevents experimental variability.

Applications in Laboratory Research

• 🔬 Protein and enzyme assays: Ensures hydrophobic molecules remain soluble for accurate measurements.
• 🧪 Buffer solutions: Prevents precipitation of reagents, ensuring stable formulations.
• 💊 Drug solubilization studies: Facilitates testing of poorly soluble compounds.

Why Choose Biofargo Tween‑80

For more information, visit the Biofargo Tween‑80 product page.