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30
Jan
NeutrAvidin Protein: Features, Stability, and Applications
Advancing High-Sensitivity Detection with Optimized Biotin-Binding Technology
NeutrAvidin protein represents the pinnacle of biotin-binding efficiency. As a deglycosylated form of avidin, it features a molecular weight of approximately 60 kDa and a near-neutral isoelectric point (pI) of 6.3.
While maintaining an ultra-high affinity for biotin ($K_a \approx 10^{15} M^{-1}$), NeutrAvidin is specifically engineered to eliminate the common pitfalls of native avidin, such as nonspecific binding to lectins and high background noise.
Key Technical Properties
Optimized Structure
Deglycosylation removes carbohydrate moieties, preventing nonspecific interactions with lectins commonly found in native avidin.
Labeling Ready
Abundant surface-exposed lysine residues allow for seamless conjugation with enzymes, fluorophores, or gold nanoparticles.
Low Background
In His-tagged protein detection, NeutrAvidin offers superior signal-to-noise ratios compared to traditional anti-His antibodies.
Exceptional Stability Profile
| Parameter | Performance Details |
|---|---|
| Thermal Stability | Resistant up to 100°C; maintains integrity in heat-intensive assays. |
| pH Range | Broad tolerance from pH 2.0 to 13.0. |
| Enzymatic Resistance | Highly resistant to proteolytic degradation, especially when bound to biotin. |
| Storage | Long-term stability at -20°C (up to 12 months). |
How it Compares
- vs. Native Avidin: NeutrAvidin eliminates the high pI (10+) and carbohydrate-related binding issues, resulting in significantly lower background.
- vs. Streptavidin: With a neutral pI of 6.3, NeutrAvidin avoids the RYD recognition sequence issues and provides a more stable alternative in neutral pH environments.
Primary Applications
- Immunodetection: ELISA, IHC, and Western Blotting.
- Molecular Biology: DNA probes, qPCR, and NGS workflows.
- Purification: Affinity chromatography for biotinylated targets.
Handling Best Practices
- Avoid repeated freeze-thaw cycles.
- Use cold-chain logistics for transport.
- Verify conjugation efficiency post-labeling to ensure protein activity remains intact.
Biofargo team
