Cell Culture Flasks (TC-Treated & Non-Treated, Sterile, Vented Cap)

Browse our selection of cell culture flasks designed for reliable cell handling in CO₂ incubators. This collection includes both TC-treated and non-treated surfaces, along with sterile packaging and vented (filter) caps to support consistent workflows across different cell culture needs.

Choose TC-treated flasks for adherent cell attachment and routine growth, or select non-treated flasks for suspension culture, mixing, and specialized applications where surface attachment is not required.

Quick Pick: Choose by Surface Type

• TC-Treated Cell Culture Flasks – for adherent cell attachment and consistent growth
• Non-Treated Cell Culture Flasks – for suspension culture and applications where low attachment is preferred

Quick Pick: Choose by Flask Format

• T25 Cell Culture Flasks (25 cm²) – starter cultures, cloning workflows, small-scale experiments 
• T75 Cell Culture Flasks (75 cm²) – routine expansion and general-purpose cell culture 
• T175 Cell Culture Flasks (175 cm²) – higher-yield growth and scale-up workflows 

How to Choose the Right Cell Culture Flask

1) Choose the Surface: TC-Treated vs Non-Treated

• TC-treated: Designed to support adherent cell attachment and consistent growth for many common adherent cell lines.
• Non-treated: Preferred for suspension culture or workflows where minimal surface attachment is desired, including mixing, storage, and specialized protocols.

2) Choose the Size: Growth Area & Throughput

Select the flask format based on your target yield, incubator space, and passaging schedule. Larger growth areas can reduce the number of vessels needed during scale-up, while smaller flasks are convenient for controlled runs.

• T25 (25 cm²): Starter cultures and small-scale experiments
• T75 (75 cm²): Routine culture and everyday expansion
• T175 (175 cm²): Scale-up and higher-yield growth

Collection Highlights

Vented Cap for Reliable Gas Exchange

Vented (filter) caps support continuous gas exchange in CO₂ incubators, helping maintain suitable culture conditions during extended incubation and routine workflows.

Angled Neck for Easier Handling

The angled neck design improves access for pipettes and cell scrapers, making feeding and harvesting steps more efficient and reducing handling errors.

Clear Polystyrene for Easy Observation

Optically clear construction supports routine microscopic observation and quick visual checks of culture condition and cell morphology.

Best-Fit Applications

• Adherent cell culture using TC-treated surfaces for attachment-dependent lines
• Suspension culture and low-attachment workflows using non-treated surfaces
• Cell expansion for downstream assays, imaging, and sample preparation
• Drug screening and toxicity assays
• General lab workflows requiring sterile, vented-cap flasks

Compare Flask Formats (T25 vs T75 vs T175)

FAQ

What is the difference between TC-treated and non-treated flasks?

TC-treated flasks are designed to support adherent cell attachment and growth. Non-treated flasks are preferred for suspension culture or workflows where minimal attachment is desired.

What does a vented (filter) cap do?

A vented (filter) cap supports gas exchange in CO₂ incubators while helping reduce contamination risk during incubation.

Which flask size should I choose?

Choose T25 for starter cultures and small-scale work, T75 for routine expansion, and T175 for scale-up and higher-yield growth.

Are these flasks sterile?

Yes, products in this collection are supplied sterile and ready to use for aseptic workflows.

Shop This Collection

Browse all cell culture flasks in this collection to find the right surface and format for your workflow. Use consistent flask formats across your lab to simplify training, inventory, and ordering.

• View All Cell Culture Flasks in This Collection