Bradford Reagent 100 mL (BDE641): The Best-Value Protein

Quantification Tool for Teaching Labs, Research Groups, and Core Facilities

Protein quantification is one of the most common and essential workflows in both life science education and research. Whether you are teaching an undergraduate biochemistry course, running daily protein purification, or operating a busy core facility, you need an assay that is fast, reliable, intuitive to use, and cost-efficient for high sample throughput.
Biofargo’s Bradford Reagent 100 mL (Sku: BDE641) delivers all of these advantages.

As a ready-to-use Coomassie dye–binding protein assay reagent, it offers high sensitivity, excellent linearity, easy operation, and significant cost savings—making it particularly well-suited for environments that require repetitive, large-volume protein measurements.

This article highlights the practical applications of Bradford reagent across teaching labs, research groups, and biotech workflows, and explains why Biofargo’s BDE641 is a standout choice.

1. Why Teaching Labs Love Biofargo Bradford Reagent

1.1 Highly visual and student-friendly

Teaching labs need experiments that are:

· Easy to demonstrate

· Intuitive for students

· Visually engaging

The Bradford reaction produces a clear and vibrant color shift from reddish-brown to deep blue upon protein binding. This makes the assay ideal for teaching:

· Protein structure

· Dye–protein interactions

· Standard curve generation

· Beer–Lambert Law

Students can see the chemistry happening—making it one of the most effective introductory quantitative biochemical assays.

1.2 Cost-effective for large class sizes

Universities often run classes of 40, 80, or even 200+ students. More expensive protein assays can quickly exhaust teaching budgets.

Biofargo’s BDE641:

· Is significantly more economical than many brand-name alternatives

· Supports hundreds of student samples per bottle

· Works perfectly with 96-well plate readers commonly used in teaching labs

For high-enrollment labs, the ability to keep costs low without compromising quality is a major advantage.

2. Daily Research Lab Applications

2.1 Ideal for routine protein extraction and Western blot sample prep

Academic research groups perform protein quantification frequently:

· Cell lysate preparation

· Western blot normalization

· Enzyme stock concentration

· Fraction analysis during purification

With a 10-minute workflow, Biofargo’s Bradford reagent fits easily into any daily experiment schedule.

2.2 Fast and robust for repeated assays

Many labs run protein quantification several times a day. BDE641:

· Requires no heating

· Requires no enzyme incubation

· Delivers results quickly

· Works with both cuvettes and microplates

This makes it perfect for high-frequency workflows.

3. A Practical Tool for Protein Purification Workflows

During His-tag, GST-tag, SEC, or ion-exchange purification, researchers need rapid concentration checks on every step:

· Crude lysate

· Flow-through

· Wash fractions

· Elution fractions

· Concentrated protein

BDE641 enables:

· Fast mid-run testing

· Reliable concentration normalization

· Clear tracking of purification performance

Its ready-to-use format ensures smooth execution without delaying purification schedules.

4. Why Core Facilities Prefer BDE641

Core facilities often process dozens to hundreds of samples per day for multiple research groups. They require:

· High reproducibility

· Batch stability

· Clear documentation

· Cost efficiency

Biofargo’s Bradford Reagent supports all of these needs:

· Strong batch consistency

· Full documentation (COA, SDS, info sheet)

· Excellent performance in 96-well throughput

· Lower cost per assay for institutional budgeting

Platforms that operate under strict documentation, compliance, and training requirements often find BDE641 to be the most seamless option.

5. For Biotech QC and R&D Laboratories

Beyond academia, biotech labs rely on Bradford assays for:

· Monitoring protein during manufacturing

· QC checks in biochemical production

· Rapid verification of protein-containing buffers or intermediates

· Routine R&D workflows requiring reproducible results

Biofargo provides:

· Full SDS and COA

· Stable U.S.-based inventory

· Bulk quotation options

· Batch-to-batch consistency

For QC labs that run protein concentration tests daily, BDE641 is a powerful, budget-friendly solution.

6. Key Technical Advantages of Biofargo Bradford Reagent 100 mL

High sensitivity

Detects as little as 0.2 µg protein.

Excellent linearity

Reliable quantification in the 10–150 µg/mL range.

Two validated assay formats

· Test tube: 100 µL sample + 1 mL reagent

· 96-well plate: 20 µL sample + 200 µL reagent

Both produce consistent, reproducible results with minimal effort.

Fast results

Only 10 minutes are needed for color development.

More economical than brand-name alternatives

Comparable to Sigma B6916 but at a much more accessible price—especially important for teaching labs and high-frequency workflows.

7. Who Should Choose Biofargo Bradford Reagent?

This reagent is an excellent fit for:

✔ Teaching labs and undergraduate biochemistry courses

· Ideal for demonstrations, student practice, and experiential learning

· Low cost supports large class sizes

✔ Academic research labs

· Daily protein quantification

· Western blot normalization

· Enzyme and lysate concentration measurements

✔ Protein purification teams

· Fraction monitoring

· Process optimization

· Rapid purity assessments

✔ Core facilities

· High-throughput quantification

· Consistent batch performance

· Low cost per assay

✔ Biotech QC and R&D teams

· Standardized protein testing

· Reliable documentation

· Stable local supply

If your laboratory runs protein assays frequently—or teaches the next generation of scientists—Biofargo’s Bradford Reagent offers an exceptional balance of quality, usability, and affordability.

8. Final Thoughts

Biofargo Bradford Reagent 100 mL (BDE641) provides a robust, fast, and economical solution for protein quantification across a wide range of environments—from university classrooms to biotech R&D labs.

With high sensitivity, excellent linearity, and a ready-to-use format, it not only simplifies workflows but also supports large-scale teaching and high-throughput sample processing. Whether you’re educating students, running a research group, or managing a core facility, BDE641 is designed to deliver consistent, reliable performance at a fraction of the cost of major commercial brands.