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Specification
| Full name | 6×His Tag rabbit polyclonal antibody |
| Reactivity | Fusion proteins containing the HIS Tag |
| Applications | WB |
| Host | Rabbit |
| Clone type | rabbit polyclonal |
| Conjugate | Unconjugate |
| Target Background |
The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells. |
| WB Predicted band size | 55 kDa |
| WB Positive control | 0.5/0.25/0.5/0.25 μg Fusion protein containing the HIS Tag |
| WB Recommended dilution | 2000-10000 |
Storage
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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