Specification

Full name	GAPDH rabbit polyclonal antibody
Alternative names	G3PD; GAPD; HEL-S-162eP
Reactivity	Human, Mouse, Rat
Applications	WB, IHC
Host	Rabbit
Clone type	rabbit polyclonal
Conjugate	Unconjugate
Target Background	This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Swissprot No	P04406
Gene Accession	BC001601
WB Predicted band size	36 kDa
WB Positive control	293T, Raji, Jurkat, 231, Hela, HUVEC, A375 cell lysates
WB Recommended dilution	5000-20000
IHC predicted cell location_1	Predicted cell location: Cytoplasm and Nucleus
IHC positive control_1	Positive control: Human breast cancer
IHC Recommed dilution	Recommended dilution: 50-200

Storage

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

GAPDH rabbit polyclonal antibody-KleanAB