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$12,800.00
Sku: BF-M2005
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by October 13, 2026
Description
Biofargo Fluorometer S96 is a compact two-channel fluorescence microplate reader for quantifying DNA, RNA, microRNA and protein. It reads a full 96-well plate in 55 seconds, so a batch that takes most of a morning on a single-sample fluorometer is finished in under a minute of instrument time.
Each well needs only 1–20 µL of sample, made up to 200 µL with the assay working solution in a 0.3 mL 96-well flat-bottom black plate. Blue and red excitation with green and red emission detection covers dsDNA, ssDNA, RNA, microRNA and protein assays on one instrument, and a SiPMT detector with top-reading optics gives a dynamic range of five orders of magnitude.
The instrument is run from PC software that handles protocol creation, well layout, sample entry, result review and Excel export, with a Chinese and English interface. Two-point calibration is used for quantification, so each plate carries its own standards rather than a stored factor.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Specifications
| Parameter | Specification |
| Product Name | Biofargo Fluorometer S96 |
| Model | BF-M2005 |
| Format | 96-well microplate, top-reading |
| Sample Throughput | Up to 96 samples per run |
| Read Time | 55 seconds per full 96-well plate |
| Sample Volume | 1–20 µL per well |
| Total Reaction Volume | 200 µL per well |
| Compatible Consumable | 0.3 mL 96-well flat-bottom black microplate, 128 × 86 × 15 mm |
| Maximum Consumable Height | 15 mm |
| Light Source | LED |
| Fluorescence Channels | 2 (blue and red excitation) |
| Blue Excitation / Green Emission | 457–483 nm / 520–540 nm |
| Red Excitation / Red Emission | 622–638 nm / 672–712 nm |
| Detector | SiPMT |
| Sample Types | dsDNA, ssDNA, RNA, microRNA, protein; RFU mode |
| Sensitivity | dsDNA 0.05 ng/µL; ssDNA 0.1 ng/µL; microRNA 0.1 ng/µL; RNA 0.5 ng/µL |
| dsDNA Detection Range | HS 0.01–100 ng/µL; BR 1–1,000 ng/µL |
| RNA Detection Range | HS 0.25–100 ng/µL; BR 1–1,000 ng/µL |
| Calibration | Two-point |
| Dynamic Range | 5 orders of magnitude |
| Software | PC software; protocol creation, well layout, result review, Excel export; Chinese and English interface |
| Communication | Bluetooth and serial port |
| Power Supply | DC 24 V, 2.5 A; 60 W maximum |
| Operating Environment | 15–30 °C, <75% RH, indoor use only |
| Dimensions / Weight | 274 × 180 × 106 mm / 2.7 kg |
| Intended Use | For research use only |
Features
96 samples per run — a full plate reads in 55 seconds, so large batches no longer go tube by tube.
1–20 µL of sample per well, so dilute lysates, eluates and small library preps can be measured without sacrificing material.
Two channels on one instrument — blue and red excitation with green and red emission covers dsDNA, ssDNA, RNA, microRNA and protein assays.
SiPMT detector with top-reading optics gives a dynamic range of five orders of magnitude.
Two-point calibration — standards are run on the same plate as the samples, so each plate carries its own calibration.
RFU mode for reading raw fluorescence intensity where no standard curve is wanted.
Excel export straight out of the PC software, so results drop into an existing analysis sheet.
Bench-friendly footprint — 274 × 180 × 106 mm and 2.7 kg, so it fits beside a workstation rather than needing its own bay.
Applications
Library QC at scale: Quantify a full plate of NGS libraries before pooling and normalization.
Nucleic acid extraction QC: Check yield across a 96-well extraction plate in one read.
Sample normalization: Equalize DNA, RNA or protein input across a plate before downstream reactions.
microRNA quantification: Measure small RNA fractions where mass is limited.
Protein quantification: Read total protein across a plate of lysates for activity normalization.
RFU measurement: Track fluorescence intensity directly without a standard curve.
Storage & Shipping
Ship and store the instrument in its original packaging.
Operate at 15–30 °C and below 75% relative humidity, indoors only.
Keep the plate chamber closed when not in use to limit dust on the optics.
Allow the instrument to stabilize for 15 minutes after power-on before the first read.
Protocol Overview
Power on the instrument and allow 15 minutes for the optics to stabilize. Connect it to the PC with the supplied cable and open the control software.
Prepare the assay working solution for the chosen Biofargo assay kit and dispense it into a 0.3 mL 96-well flat-bottom black microplate.
Add the kit standards to their wells, then add 1–20 µL of each sample to its well and bring every well to a total volume of 200 µL.
Mix and incubate as the assay kit specifies, protected from light.
In the software, create or select the protocol for the assay, lay out the standard and sample wells, and enter the sample information.
Load the plate and start the run. A full plate reads in 55 seconds.
Review the standard curve and the results on screen, then export to Excel. Dilute and re-read any sample that falls above the top standard.
Frequently Asked Questions (FAQs)
Common questions about Biofargo Fluorometer S96.
How many samples can the S96 measure at once?
Up to 96, using a 0.3 mL 96-well flat-bottom black microplate. A full plate reads in 55 seconds.
How does the S96 compare with a single-sample fluorometer?
A single-sample fluorometer such as the Biofargo Fluorometer X1 reads one tube at a time, which suits a handful of samples. The S96 reads a whole 96-well plate in 55 seconds, so it is the right choice once batches are routinely larger than a few dozen samples. The eight-position Biofargo Fluorometer X3 sits between the two.
What assay kits does it run?
It runs the Biofargo fluorometric assay kits for dsDNA (HS, BR and 1X HS), RNA (HS and BR), ssDNA and protein. The Compatible Assay Kits table on this page lists each kit with its analyte and assay range.
How much sample is required per well?
Each well takes 1–20 µL of sample, made up to a total volume of 200 µL with the assay working solution. Choose the input volume so the target mass falls within the kit's assay range.
Which microplate should I use?
A 0.3 mL 96-well flat-bottom black microplate measuring 128 × 86 × 15 mm. The consumable height must not exceed 15 mm. Black plates limit well-to-well crosstalk and background.
Does it need a computer?
Yes. The instrument is run from PC software over the supplied cable. The software handles protocol creation, well layout, sample information, the run itself, result review and Excel export, and its interface is available in Chinese and English.
What is the sensitivity and detection range?
Sensitivity is 0.05 ng/µL for dsDNA, 0.1 ng/µL for ssDNA and microRNA, and 0.5 ng/µL for RNA. Detection ranges are 0.01–100 ng/µL for dsDNA HS, 1–1,000 ng/µL for dsDNA BR, 0.25–100 ng/µL for RNA HS and 1–1,000 ng/µL for RNA BR.
How is the instrument calibrated?
Quantification uses two-point calibration with the standards supplied in the assay kit. Run the standards on the same plate as the samples in every batch rather than relying on a stored curve.
Can it read raw fluorescence without a standard curve?
Yes. RFU mode reports fluorescence intensity directly, which is useful for relative measurements and for checking a new assay before building a curve.
How long does it take to stabilize after power-on?
Allow 15 minutes after power-on before the first read.
Can it be used for clinical or diagnostic purposes?
No. This product is for research use only and is not intended for human, diagnostic or therapeutic use.
Compatible Assay Kits
The following Biofargo fluorometric assay kits run on this instrument. Select the kit by analyte and expected mass range.
| Assay Kit | Analyte | Assay Range |
| Biofargo dsDNA HS Assay Kit | Double-stranded DNA | 0.1–120 ng |
| Biofargo 1X dsDNA HS Assay Kit | Double-stranded DNA (ready-to-use 1X) | 0.2–100 ng |
| Biofargo dsDNA BR Assay Kit | Double-stranded DNA | 2–1,000 ng |
| Biofargo RNA HS Assay Kit | RNA | 5–100 ng |
| Biofargo RNA BR Assay Kit | RNA | 20–1,000 ng |
| Biofargo ssDNA Assay Kit-V2 | Single-stranded DNA | 1–200 ng |
| Biofargo Fluorometric Protein Quantification Kit | Protein | 0.25–5 µg |
Lower-throughput alternatives: Biofargo Fluorometer X1 (single sample) and Biofargo Fluorometer X3 (eight sample positions).
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
