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$22.00
Sku: BF-T1001-S
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by October 13, 2026
Description
The Biofargo dsDNA HS Assay Kit provides fluorescence-based quantification of double-stranded DNA (dsDNA) for molecular biology research. Selective for dsDNA over RNA.
The total target amount is 0.1–120 ng per assay. Use 1–20 µL of sample in a 200 µL final reaction and incubate for 2 minutes at 20–25 °C, protected from light. Prepare fresh working solution by mixing 1 volume of fluorescent reagent with 199 volumes of the supplied buffer (1:200 final dilution).
Available formats: BF-T1001-S (20 reactions) and BF-T1002 (500 reactions). For research use only. Not for diagnostic or therapeutic procedures.
Specifications
| Parameter | Specification |
| Biofargo catalog numbers | BF-T1001-S / BF-T1002 |
| Target | double-stranded DNA (dsDNA) |
| Total target amount per assay | 0.1–120 ng |
| Initial sample concentration | 0.005–120 ng/µL (5 pg/µL at the lower end), depending on sample input |
| Sample input | 1–20 µL |
| Final assay volume | 200 µL per tube or well |
| Working-solution preparation | Prepare fresh working solution by mixing 1 volume of fluorescent reagent with 199 volumes of the supplied buffer (1:200 final dilution). |
| Incubation | 2 minutes at 20–25 °C, protected from light |
| Signal stability | Up to 3 hours, protected from light |
| Instrument formats | Biofargo X1 and X3; Qubit fluorometers with the matching assay; fluorescence microplate readers |
| Plate-reader settings | 485 nm excitation / 530 nm emission |
| Available formats | BF-T1001-S: 20 reactions; BF-T1002: 500 reactions |
| Intended use | Research use only |
Features
Concentrated fluorescent reagent and assay buffer support freshly prepared working solution.
Quantifies double-stranded DNA (dsDNA) at 0.1–120 ng per assay.
Selective for dsDNA over RNA.
Uses 1–20 µL of sample in a 200 µL reaction.
Two-minute incubation at room temperature.
Supports tube-based and fluorescence microplate-reader workflows.
Applications
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Extracted nucleic acids: Determine sample concentration after purification and before downstream molecular biology workflows.
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NGS sample preparation: Measure target nucleic acid concentration for input normalization; this concentration assay does not measure fragment size, integrity or amplifiable library concentration.
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Fluorescence microplates: Use a black 96-well plate and a multi-point standard curve at 485 nm excitation / 530 nm emission.
Kit Components
| Component | Designation | BF-T1001-S (20 reactions) | BF-T1002 (500 reactions) | Function |
| dsDNA HS Buffer QB | QB | 10 mL | 250 mL | Working-solution buffer |
| dsDNA HS Standard QS0 | QS0 | 200 µL | 5 mL | Blank standard; 0 ng/µL |
| dsDNA HS Standard QS1 | QS1 | 200 µL | 5 mL | High standard; 10 ng/µL |
| dsDNA HS Fluorescent Reagent QF | QF | 50 µL | 1.25 mL | Concentrated detection reagent |
Storage & Shipping
Ship on ice.
Store all components at 2–8 °C, protected from light.
Frequently Asked Questions (FAQs)
What is the quantification range?
The target mass range is 0.1–120 ng in the assay. The stated initial concentration range is 0.005–120 ng/µL (5 pg/µL at the lower end), depending on sample input. Initial sample concentration is measured before dilution; target mass equals initial concentration multiplied by the sample volume. These are different quantities.
How do I prepare the working solution?
Prepare fresh working solution by mixing 1 volume of fluorescent reagent with 199 volumes of the supplied buffer (1:200 final dilution).
Can I use this kit with Qubit fluorometers?
The supplier protocol supports use with the matching Qubit assay program. Use this kit's own standards, the specified sample volume and the correct consumables for the Qubit model. Compatibility does not imply Thermo Fisher endorsement or identical performance. For the 1X assay on older Qubit models, verify that the appropriate assay program is installed.
Which Biofargo fluorometers support this assay?
X1 and X3 list the corresponding dsDNA or ssDNA program. The eight-position X3 uses different tubes from the single-sample X1.
How much sample is required?
Add 1–20 µL of sample and adjust the working solution to a final volume of 200 µL. Choose the input volume so the target mass lies within the assay range. Dilute samples that exceed the upper limit.
Which assay tubes should I use?
Use clear, thin-wall 0.5 mL assay tubes for Biofargo X1 and Qubit 2/3/4. Use the instrument-specified clear 0.2 mL tubes or eight-tube strips for Biofargo X3; Qubit Flex requires its specified 0.2 mL assay tube strips. Keep the total assay volume at 200 µL.
How long does the assay take?
Incubate for 2 minutes at 20–25 °C, protected from light, after mixing. This is the incubation time, not the total preparation and measurement time. The signal remains stable for up to 3 hours when protected from light.
Do I need new standards?
Use the two standards supplied with this kit and the matching assay program. A new calibration for each run is recommended. Do not reuse a calibration generated with a different kit. For a plate reader, prepare a dilution series and fit a standard curve.
Does the reaction count equal the number of unknown samples?
The listed count is the nominal assay format. Standards, controls, repeat measurements and handling losses reduce the number of unknown samples that can be measured.
How should I store the kit?
Ship on ice. Store all components at 2–8 °C, protected from light.
Is this product intended for diagnostic use?
No. For research use only. Not for diagnostic or therapeutic procedures.
Compatible Instruments
This kit is run on the Biofargo fluorometers below.
Biofargo Fluorometer X1 — Single sample position. Select the matching assay program on the instrument.
Biofargo Fluorometer X3 — Eight sample positions. Select the matching assay program on the instrument.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
