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$5,523.00
Sku: BF-M2003
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by October 13, 2026
Description
The Biofargo Fluorometer X3 (BF-M2003) is an eight-position fluorometer for fluorescence-based quantification using matched assay reagents. It supports dsDNA HS, dsDNA BR, RNA HS, RNA BR, RNA XR, ssDNA, microRNA and Protein BR.
Use 1–20 µL of sample with the reaction volume specified by the selected assay. The instrument has a 5-inch touchscreen, two-point calibration and USB data export. The specified read time is no more than 5 seconds per sample; this excludes sample preparation and incubation.
Assay reagents and tubes are purchased separately. For research use only. Not for diagnostic or therapeutic procedures.
Specifications
| Parameter | Specification |
| Biofargo catalog number | BF-M2003 |
| Sample positions | 8 |
| Sample input | 1–20 µL; depends on assay |
| Assay volume | 200 µL for the listed nucleic acid protocols; follow the selected assay instructions |
| Compatible tubes | Instrument-specified clear 0.2 mL PCR single tubes or eight-tube strips; flat, straight caps required |
| Read time | ≤5 seconds per sample; not a stated eight-sample batch time |
| Optical channels | Blue (460–480 nm excitation / 500–535 nm emission) and red (630–650 nm excitation / 670–710 nm emission) |
| Detector | SiPMT (silicon photomultiplier) |
| Assay programs | dsDNA HS, dsDNA BR, RNA HS, RNA BR, RNA XR, ssDNA, microRNA and Protein BR |
| Calibration | Two-point assay calibration using matched standards |
| Display | 5-inch touchscreen |
| Data export | USB flash drive |
| Instrument power | DC 5 V, 2 A; maximum 10 W |
| Operating conditions | Indoor use; 15–30 °C; relative humidity ≤75%; altitude up to 2,000 m |
| Transport/storage temperature | −20 to 60 °C |
| Weight | 0.5 kg |
| Intended use | Research use only |
| Data storage | 8 GB; up to 20,000 records |
Features
Eight individually selectable sample positions with clear 0.2 mL tubes.
Blue and red optical channels support DNA, RNA and Protein BR assays.
Low sample input of 1–20 µL for supported assays.
Touchscreen operation and two-point calibration.
USB export supports experiment data review.
RFU measurement and kinetics modes for fluorescence research; select the channel supported by this model.
Applications
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DNA quantification: Use matching dsDNA HS, dsDNA BR or ssDNA reagents for purified nucleic acid samples and input normalization.
-
RNA quantification: Use matching RNA HS, BR or XR assays for RNA concentration measurement. Concentration measurement does not establish RNA integrity.
-
microRNA research: Use a dedicated microRNA assay and its corresponding program; routine RNA HS/BR reagents are not interchangeable.
-
Protein quantification: Use compatible Protein BR reagents and their assay instructions. Protein BR target mass is expressed in µg, not ng; usable range is assay dependent.
-
Fluorescence research: Measure raw fluorescence or monitor fluorescence over time with the available optical channel(s).
Frequently Asked Questions (FAQs)
How does X3 compare with Qubit fluorometers?
X1 is a single-sample instrument, while X3 provides eight sample positions. Qubit 4 is also a single-sample instrument and Qubit Flex measures up to eight samples. Optical filters, consumables, software, assay menus and validation differ; matching sample capacity does not establish equivalent analytical performance.
Which reagents can I use?
The technical guide lists Biofargo BF-T1002, BF-T1004, BF-T1005, BF-T1006, BF-T1007 and BF-T1008-V2 and selected Qubit reagents. Use the matching assay program, kit standards and instrument-specified tubes. Compatibility is based on the supplier guide, not Thermo Fisher endorsement.
Which tubes does X3 require?
Instrument-specified clear 0.2 mL PCR single tubes or eight-tube strips; flat, straight caps required. Use the tube geometry specified in the technical guide; the 0.5 mL tubes used by X1 cannot be substituted for X3 tubes.
Is the read time the total assay time?
No. The specification is ≤5 seconds per sample. Preparation, calibration and incubation add time. It does not establish that eight samples are measured together in five seconds.
How do I calibrate the assay?
Select the appropriate program and read the two standards supplied with the chosen kit. For the listed nucleic acid assays, prepare each standard with 10 µL of standard and 190 µL of working solution. Enter the correct sample input volume before reading unknowns. Do not mix standards or calibration curves from different kits.
Can I export results?
Yes. Insert a USB flash drive and use the data-query/export function described in the technical guide.
What is included with the instrument?
The technical packing list includes the fluorometer, power cord, USB drive with user guide and a packing list. Assay reagents and assay tubes are separate purchases.
Is the instrument intended for diagnostic use?
No. For research use only. Not for diagnostic or therapeutic procedures.
Compatible Assay Kits
The following Biofargo fluorometric assay kits run on this instrument. Select the kit by analyte and expected mass range.
| Assay Kit | Analyte | Assay Range |
| Biofargo dsDNA HS Assay Kit | Double-stranded DNA | 0.1–120 ng |
| Biofargo 1X dsDNA HS Assay Kit | Double-stranded DNA (ready-to-use 1X) | 0.2–100 ng |
| Biofargo dsDNA BR Assay Kit | Double-stranded DNA | 2–1,000 ng |
| Biofargo RNA HS Assay Kit | RNA | 5–100 ng |
| Biofargo RNA BR Assay Kit | RNA | 20–1,000 ng |
| Biofargo ssDNA Assay Kit-V2 | Single-stranded DNA | 1–200 ng |
| Biofargo Fluorometric Protein Quantification Kit | Protein | 0.25–5 µg |
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
