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PLX-5622 is a highly selective brain-penetrant CSF1R inhibitor (IC50=0.016 µM; Ki=5.9 nM) allowing for extended and specific microglial elimination, preceding and during pathology development. Notice: PLX-5622 also works well by intraperitoneal injection (IP), See: Cell Rep. 2022 Aug 16;40(7):111209. Microglia contribute to the postnatal development of cortical somatostatin-positive inhibitory cells and to whisker-evoked cortical activity. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9396528/ Method details Microglia depletion in postnatal pups PLX5622 stock was dissolved in DMSO at a final concentration of 100mg/mL. Pups were injected intraperitoneally every day, from P2 until the day before brain collection, with 0.5μL/g of PLX5622 stock solution diluted in at least an equal volume of DMSO (PLX5622 final concentration is 50μg/g of mouse). PLX-5622 was purchased from Chemgood.
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