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Description
This kits intended for quantitative determination of kanamycin residues in plasmid DNA, that is used in the development of cell and gene therapies.
The SHENTEK® Kanamycin ELISA Kit utilizes an "indirect-competitive" enzyme immunoassay. The microplate wells are pre-coated with kanamycin antigen, which competes with kanamycin residues in the sample for binding to the anti-kanamycin monoclonal antibody. An enzyme-labeled secondary antibody then targets the primary monoclonal antibody that has formed complexes with the kanamycin antigen coated on the plate wells. Upon addition of the TMB substrate, the resulting color intensity measured at 450 nm exhibits an inverse relationship with the concentration of kanamycin residues in the sample. The total amount of kanamycin in the sample is obtained by comparison with a standard curve, and adjusted by the sample dilution factor.
For the detection of residual kanamycin in other biologics samples, sample suitability test is recommended to assess the effects of matrix interference.
| Components | Shipping Condition | Unit Size | Detection Method |
| Kanamycin Standard | 2-8℃, protect from light | 96 Tests | Sandwich ELISA |
| Kanamycin Standard Reconstitution Diluent | 2-8℃ | ||
| Kanamycin Standard Solution Diluent | 2-8℃ | ||
| 100×Kanamycin Primary Antibody | 2-8℃ | ||
| 100×Enzyme-conjugated Secondary Antibody | 2-8℃, protect from light | ||
| Kanamycin-coated Plate | 2-8℃, protect from light | ||
| 10×Wash Buffer | 2-8℃ | ||
| Chromogenic Substrate for Kanamycin ELISA | 2-8℃, protect from light | ||
| Stop Solution | 2-8℃ | ||
| Sealing Film | 2-8℃ |
Specifications
| Linearity & Range | 0.5-32 ng/mL, R2>0.990 |
| Accuracy | Recovery Rate= 80.8%-112.5% |
| LLOQ | 0.5 ng/mL |
| ULOQ | 16 ng/mL |
| Repeatability | 5.3%-9.1% (CV≤20%) |
| Specificity | No cross-reactivity with tetracycline, streptomycin sulfate, ampicillin, and chloramphenicol |
| Robustness | Incubate at 22℃ to 30℃, CV≤ 20% |
| Instrument suitability not limited to Thermo Multiskan FC, Bio-Tek Synergy2 |
Related Products
SHENTEK® Residual Host Cell DNA Sample Preparation Kit
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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