Human Serum Albumin (HSA) ELISA Kit (One-step ELISA) SHENTEK®

Description

This kit is based on the solid-phase enzyme-linked immunosorbent assay (ELISA), utilizing a double antibody sandwich approach to detect the content of Human Serum Albumin (HSA) in samples.

This kit employs a solid-phase Enzyme-linked Immunosorbent Assay (ELISA) with a double-antibody sandwich technique to detect Human Serum Albumin (HSA) in the sample. Polyclonal antibody specific to HSA was employed in the assay to capture any HSA in the samples. Both the Calibration Standards (or test samples) and the HRP (Horseradish Peroxidase) labeled with anti-HSA antibody were simultaneously added to the pre-coated microtiter plate, and followed by incubation and washing steps. Then TMB (3,3',5,5'-tetramethylbenzidine) substrate was added into reaction, HRP catalyzed the oxidation of TMB by H₂O₂ to produce a blue colored product (maximum absorption peak at 655 nm). Afterwards the stop solution was added to terminate the enzymatic reaction, resulting in a yellow colored product (maximum absorption peak at 450 nm). The absorbance values at 450 nm wavelength were positively correlated with the HSA concentration in the Calibration Standards and the samples. The concentration of HSA in the samples can be calculated using a dose-response curve.

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