Precast-Gel Hepes-Tris Native-PAGE 4-20% 10Well

$88.00

FREE
SHIPPING

100% MONEY
BACK GUARANTEE

ONLINE
SUPPORT 24/7

Sku: P310056-10 PCS
Tags:
Bulk Quotation Request

Description

Precast-GLgel Hepes Native-PAGE 4–20% (10-Well) is a ready-to-use, non-denaturing gradient gel designed for analyzing proteins in their native, biologically active conformations. The gel is built with an innovative glass plate design that minimizes non-specific protein adsorption and provides sharper, clearer native protein bands.

This 4–20% HEPES-based precast gel is supplied fully prepared with matched running buffer, allowing direct sample loading without manual gel preparation. The wide gradient range makes it suitable for resolving a broad spectrum of native protein sizes, including large molecular weight protein complexes and membrane protein complexes, which require gentle electrophoresis conditions to preserve native structure.

Specification

Full name Precast-GLgel Hepes Native-PAGE 4–20% 10-Well
Package 10 pcs
Components 10 pcs gel, 10 pcs Native-PAGE running buffer
Gel size 8 × 7.4 × 0.15 cm
Plate size 9.8 × 8.4 × 0.41 cm
Wells 10 wells
Buffer system HEPES buffer system
Maximum sample loading volume 60 µL
Recommended sample loading volume 30 µL

 

Buffers

Buffer Hepes Running Buffer for Native PAGE (Powder)
Specification 10*500 mL
Introduction Hepes Running buffer is formulated for separation of proteins in their denaturing or nondenaturing state on Hepes-Tris gels. This product is available in powder form, a total of 10 packs, each pack can prepare 500 mL of 1× working solution.
Protocol (for reference only)
  • Take 1 pack of powder that can prepare 500 mL of hepes running buffer, pour it into a clean beaker, add 400 mL distilled water and stir to dissolve the powder completely.
  • Make up to 500 mL in the graduated cylinder with distilled water, which is 1× working solution and can be used after mixing.
  • Unused running buffer can be stored at room temperature, usually 2-3 days at room temperature and 1-2 weeks at 4°C.
Notes
  • 1× working buffer should not be used for more than 2 weeks after preparation.
  • This running buffer is suitable for Hepes-Tris gels, and should not be used for gels with other chemistries.
  • For research use only. Not for use in diagnostic procedures.
  • For your safety and health, please wear personal protective equipment and clothing when operating.
Storage Store at room temperature, valid for 2 years.

 

Features

  • Glass plate construction minimizes non-specific protein adsorption, producing sharper and clearer native bands.

  • Automated gel filling technology ensures excellent stability and reproducibility across batches.

  • 4–20% gradient gel provides broad-range separation for diverse native protein sizes.

  • Ideal for large molecular weight native proteins and protein complexes, which benefit from the gentle separation conditions of the HEPES system.

  • Well-suited for membrane proteins and membrane protein complexes, supporting analysis while preserving native conformation.

  • SDS-free gel matrix, fully compatible with non-denaturing electrophoresis (Native-PAGE) and downstream native studies.

  • Precast and ready to use, eliminating manual gel preparation and significantly reducing experiment time.

  • Long shelf life, stored conveniently at 2–8°C.

Applications

  • Native-PAGE analysis of proteins in non-denatured form

  • Separation of large molecular weight native proteins

  • Electrophoresis of membrane proteins and membrane protein complexes

  • Analysis of native protein complexes (e.g., oligomers, assemblies)

  • Western blot (WB) after native separation

  • Experiments requiring a 4–20% native gradient gel

Storage

2~8°C, do not freeze

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

Terms and Conditions