Precast-Gel Hepes-Tris Native-PAGE 7.5% 10Well

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Sku: P310051-10 PCS
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Description

Precast-GLgel Hepes Native-PAGE 7.5% (10-Well) is a ready-to-use, non-denaturing gel designed for the analysis of proteins in their native, biologically active state. Constructed with an innovative glass plate design, it minimizes non-specific protein adsorption and delivers sharp, clear protein bands.

This 7.5% HEPES-based precast gel comes fully prepared with matched Native-PAGE running buffer, allowing direct sample loading without manual gel preparation. The 7.5% concentration is optimized for resolving larger molecular weight native proteins, including membrane proteins and native protein complexes, maintaining structural integrity while enabling gentle separation.

Specification

Full name Precast-GLgel Hepes Native-PAGE 7.5% 10-Well
Package 10 pcs
Components 10 pcs gel, 10 pcs Native-PAGE running buffer
Gel size 8 × 7.4 × 0.15 cm
Plate size 9.8 × 8.4 × 0.41 cm
Wells 10 wells
Buffer system HEPES buffer system
Maximum sample loading volume 60 µL
Recommended sample loading volume 30 µL

 

Buffers

Buffer Hepes Running Buffer for Native PAGE (Powder)
Specification 10*500 mL
Introduction Hepes Running buffer is formulated for separation of proteins in their denaturing or nondenaturing state on Hepes-Tris gels. This product is available in powder form, a total of 10 packs, each pack can prepare 500 mL of 1× working solution.
Protocol (for reference only)
  • Take 1 pack of powder that can prepare 500 mL of hepes running buffer, pour it into a clean beaker, add 400 mL distilled water and stir to dissolve the powder completely.
  • Make up to 500 mL in the graduated cylinder with distilled water, which is 1× working solution and can be used after mixing.
  • Unused running buffer can be stored at room temperature, usually 2-3 days at room temperature and 1-2 weeks at 4°C.
Notes
  • 1× working buffer should not be used for more than 2 weeks after preparation.
  • This running buffer is suitable for Hepes-Tris gels, and should not be used for gels with other chemistries.
  • For research use only. Not for use in diagnostic procedures.
  • For your safety and health, please wear personal protective equipment and clothing when operating.
Storage Store at room temperature, valid for 2 years.

 

Features

  • Glass plate construction minimizes non-specific protein adsorption, producing sharper and clearer protein bands.

  • Automatic gel filling technology ensures consistent quality and excellent batch-to-batch reproducibility.

  • 7.5% acrylamide concentration provides optimal resolution for larger molecular weight native proteins.

  • Suitable for membrane proteins and membrane protein complexes, preserving structural integrity under non-denaturing HEPES conditions.

  • Supports separation of native protein complexes, including oligomers and multi-subunit assemblies.

  • SDS-free gel matrix, ideal for true Native-PAGE studies and downstream functional analyses.

  • Ready-to-use precast format eliminates manual gel preparation, saving significant laboratory time.

  • Long shelf life with convenient storage at 2–8°C.

Applications

  • Native-PAGE analysis of proteins in non-denatured form

  • Separation of larger molecular weight native proteins

  • Electrophoresis of membrane proteins and membrane protein complexes

  • Analysis of native oligomers and protein complexes

  • Western blot (WB) after native separation

  • Experiments requiring a 7.5% native gel

Storage

2~8°C, do not freeze

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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