Microporous Membranes for Blotting Proteins and Nucleic Acids

Description

BIO BASIC's NYLON microporous membranes are solid phase supports with high binding characteristics for biologically active molecules. They provide high surface area matrices for immobilization of nucleic acids and proteins and are ideal for most applications requiring nitrocellulose or nylon membranes. Membranes are also very durable unlike nitrocellulose they will not tear, crack, or curl during handling. This permits convenient re-probing and easy removal of target bands for subsequent analysis.

BIO BASIC’s transfer membranes have a high affinity for proteins and/or nucleic acids. The capacity 
and binding strength of the NYLON membranes require much smaller quantities of these biomolecules for signal detection than standard nitrocellulose membranes. These membranes provide an available immobilization area of up to 500 sq. cm per sq. cm of surface area facilitating the immobilization of up to 100 mg/sq. cm of DNA and 200 mg/sq. cm of protein. They also provide a low background in radioactive and non-radioactive detection systems resulting in clear, accurate signal detection.

NYLON Membranes 

This positively charged membrane provides higher sensitivities and rapid nucleic acid binding without the need for UV crosslinking or baking. The surface chemistry is characterized by a high density of strongly cationic quaternary ammonium groups which are an integral part of the membrane. The NYLON membranes are ideal for superior binding of negatively charged biomolecules in a wide variety of nucleic acid and protein applications.

Applications 

Northern, Southern and Western Blotting 

Dot/Slot Blots 

Membrane Bound PCR 

Colony Hybridization

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Disclaimer: For laboratory research use only.