Synonyms: BM Blue, SureBlue TMB, TMB Substrate, TMB Blotting Plus

Description

3,3',5,5'-Tetramethylbenzidine (TMB) Sensitive ELISA Substrate Solution — a high-purity, ready-to-use colorimetric substrate for HRP (horseradish peroxidase)-based immunoassays. This premium TMB reagent delivers consistent, high-sensitivity color development in ELISA, western blot and other peroxidase analytical applications. Upon oxidation by HRP and hydrogen peroxide, the solution produces a blue chromogenic product measurable at 370 nm or 655 nm, which shifts to yellow at 450 nm when terminated with acid for accurate quantification. Ideal for research laboratories demanding high reproducibility, rapid results and compatibility with automated plate readers.

Keywords: TMB substrate, ELISA chromogenic reagent, HRP color development, high sensitivity, ready-to-use.

Specifications

Grade	High Purity
Formula	C16H20N2
C16H20N2	240.4
CAS	54827-17-7
Appearance	White or light yellow crystal
Assay (HPLC)	≥99.0%
Melting point	168.0 – 171.0℃
TLC	One spot
Heavy metals	≤5ppm
Loss on drying	≤0.5%
Storage	4℃

Features

High Purity & Reliable Performance — ≥99% pure 3,3’,5,5’-tetramethylbenzidine ensures robust, reproducible colorimetric results for sensitive HRP assays.
Ready-to-Use Chromogenic Substrate — no preparation needed; simplifies workflow for ELISA, WB and immunoassay applications.
Enhanced Sensitivity & Dynamic Range — strong blue color development with excellent absorbance at 370/655 nm, shifting to 450 nm after acid stop.
Versatile Compatibility — ideal for HRP-conjugated antibody systems, microwell plate assays, membrane blotting and automated detection systems.
Stable & Lab-Grade Quality — suitable for research use, consistent across batches, minimizing variability in quantitative assays.

Applications

ELISA (Enzyme-Linked Immunosorbent Assay) — quantitative and qualitative immunoassays using HRP conjugates.
Western Blot / Immunoblot Detection — chromogenic substrate for membrane-based protein visualization.
Peroxidase Activity Screening — HRP reporter assays requiring colorimetric readouts.
Biochemistry Labs & Diagnostic R&D — research applications involving TMB-HRP systems.
Compatible with automated plate readers and spectrophotometers for OD measurement at 450 nm, 655 nm, 370 nm.

Documents

COA

SDS

Disclaimer: For laboratory research use only.

3,3',5,5'-Tetramethylbenzidine 99% High Purity Grade