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$106.00
Sku: TL-302-0010
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by March 13, 2026
Description
Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is a pivotal glycosylated cytokine functioning extracellularly as a homodimer. Its core function is to stimulate the proliferation and differentiation of hematopoietic stem/progenitor cells into granulocytes and monocytes (which mature into macrophages and dendritic cells).
Leveraging this, GM-CSF finds extensive application in cell and gene therapy. In cell therapy, it is a critical factor for the in vitro expansion of immune cells (e.g., dendritic cells for DC vaccines) and acts as an immune modulator in vivo to activate DCs and T cells, enhancing anti-tumor immunity.
In gene therapy, its encoding gene (located in the chromosome 5q31 region) serves as a potential target, or therapeutic GM-CSF can be delivered via vectors for localized and sustained expression.
Recombinantly produced GM-CSF protein provides an essential tool for these applications, with its potential dual immunomodulatory effects requiring careful evaluation.
Specifications
| Product Details | ||
|---|---|---|
| Parameter | RUO Grade | GMP Grade |
| Catalog Number | TL-302-0010 | GMP-TL302-0100 |
| Synonyms | CSF2, MGC131935 | Colony-stimulating factor (CSF), Molgramostin, Sargramostim, CSF2 |
| Accession | UniProt P04141 | UniProt P04141 |
| Expressed Region | Ala18–Glu144 | Ala18–Glu144 |
| Tag | C-terminal 6×His-tag | C-terminal 6×His-tag |
| Expression System | HEK293 cells | HEK293 cells |
| Molecular Weight | 19.3 kDa | 15.3 kDa |
| Purity | >90% (SDS-PAGE) | >95% (SDS-PAGE) |
| Endotoxin | <0.1 EU/µg | <0.1 EU/µg |
| Biological Activity | TF-1 cell proliferation assay, ≥ 5.0 × 106 IU/mg |
TF-1 cell proliferation assay, ≥ 5.0 × 106 IU/mg |
| Formulation | Lyophilized from PBS (pH 7.4), 6% mannitol | Lyophilized from PBS (pH 7.4), 6% mannitol |
Features
-
✔ High Purity: Purified using advanced chromatographic techniques, this product achieves a purity level of over 95%, ensuring high reliability and reproducibility of experimental results.
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✔ High Biological Activity: Validated through multiple activity assays, this GM-CSF protein effectively stimulates target cells, supporting a variety of research applications related to immune cell function and hematopoiesis.
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✔ Batch Consistency: Produced with strict quality control measures to ensure consistent quality between batches, minimizing variability and enhancing the reliability of research outcomes.
- ✔ Animal-Free: Manufactured without animal-derived components, complying with animal-free standards and broadening its applicability for various research needs.
Applications
Hematology
Study the role of GM-CSF in the regulation of blood cell production, including granulocytes and macrophages, providing insights into hematopoietic disorders and therapies.
Immunology
Investigate GM-CSF’s effects on immune cell development and activation, contributing to a deeper understanding of immune responses and potential therapeutic applications.
Cancer Research
Examine the role of GM-CSF in tumor microenvironments and its impact on immune cell function, offering insights into cancer immunotherapy and treatment strategies.
Cell Therapy
Utilize GM-CSF to enhance the function and expansion of immune cells in cell-based therapies, advancing research in regenerative medicine and immunotherapy.
Stability & Storage
RUO
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Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored in the original sealed container under desiccant.
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Reconstitution: Dissolve contents in 200 µL sterile water for injection, 0.9% NaCl, or PBS (pH7.4), maintaining a final concentration about 50 µg/mL.
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Handling: Aliquot to avoid repeated freeze-thaw cycles.
GMP
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Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored in the original sealed container under desiccant.
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Reconstitution: Dissolve in sterile water for injection, 0.9% NaCl, or PBS (pH7.4), maintaining a final concentration ≥ 100 µg/mL to prevent adsorption.
-
Handling: Aliquot to avoid repeated freeze-thaw cycles.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Follow recommended storage and handling instructions to maintain product stability.
• Support: Contact technical support if assistance is required.
Document
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
