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Description
Protein-Free Rapid Blocking Buffer is a non-protein-based compound that provides effective blocking for membrane- and plate-based protein detection methods, resulting in extremely low background.
Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal.Protein-Free Rapid Blocking Buffer contains no endogenous phosphorylated proteins or biotin, resulting in no interference in these detection systems.The blocking buffer does not produce non-specific binding to the protein, blocking only the membrane without shielding the antibody recognition sites on the sample proteins. This greatly improves antibody detection sensitivity.
Features
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Rapid And Efficient:
Complete blocking in 5-10 minutes.
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Protein Free:
No cross-reactivity with antibodies or target proteins, higher signal-noise ratio compared to non-fat milk and BSA. Suitable for the detection for phosphoproteins.
Applicable to phosphorylated proteins

Applicable to phosphorylated proteins

Specifications
Product Type | Blocking Buffer |
Form | Liquid |
Application | Western Blot |
Concentration | 1× |
Quantity | 500 mL |
Storage
Transported at room temperature and stored at 4°C. The shelf life is 12 months.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.