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Empty columns with cap & stopper¡ªreliable small-scale purification
Description
The 20 mL Gravity Columns (total volume 32 mL) are economical, disposable tools designed for small-scale gravity flow protein and antibody purification, buffer exchange, and desalting. Supplied empty (no resin included), they feature leak-proof screw caps, durable polyethylene frits, and clear graduations¡ªdelivering flexibility, reliability, and cost-effective performance. Fully compatible with LabMate-style racks, these columns provide researchers with a convenient solution for rapid sample cleanup prior to SDS-PAGE, HPLC, or mass spectrometry.
Specification
| Parameter | Specification |
|---|---|
| Package | 5 PCS/25/PK |
| Full name | Gravity Columns, 20 ml |
| Composition | Columns, matching cap, frits, and rubber stoppers. |
| Total Volume | 32 ml |
| Resin Volume | 20 ml |
| Reservoir Volume | 12 ml |
| Closure | Plastic Stopper |
| Cap | Screw cap |
| Frit | 3 mm ~30 ¦Ìm hydrophobic Polyethylene. |
| Component | Columns, matching cap, frits, and rubber stoppers. |
Features
- Total Volume: 32mL
- Resin Volume: 20mL
- Reservoir Volume: 12mL
- Graduated Closure: Plastic Stopper
- Cap: Screw cap
- Frit: 3mm ~30¦Ìm hydrophobic polyethylene
Applications
small-scale protein and antibody purification, buffer exchange, desalting, and rapid sample cleanup before downstream analysis such as SDS-PAGE, HPLC, or mass spectrometry.
Gravity Columns, 20 mL ¨C FAQ
Q: What are they used for?
A: Perfect for small-scale protein and antibody purification, buffer exchange, and quick sample cleanup before downstream analysis.
Q: What samples can I load?
A: Typically cell lysates, fermentation supernatants, or antibody-containing culture media.
Q: What quality matters most?
A: Leak-proof screw caps, stable frits for uniform flow, and clean, transparent columns ensure reliable and reproducible results.
Q: Any common challenges?
A: Flow control and recovery can be tricky, but our well-designed frits and column geometry minimize dead volume and sample loss.
Q: Why choose these columns?
A: They¡¯re clean, consistent, and cost-effective¡ªwith multiple pack sizes (5 pcs / 25 pcs) for flexible lab use.
Q: Do these columns fit LabMate-style racks?
A: Yes¡ªour 20 mL gravity columns fit most LabMate-style and universal chromatography racks designed for ~18 mm wells. If your rack uses special-size wells, please check the well diameter before purchase.
Q: Can I collect into 15 mL or 50 mL tubes?
A: Yes. The outlet geometry allows stable positioning over both 15 mL and 50 mL conical tubes.
Q: Are these compatible with ion-exchange/affinity/gel-filtration resins?
A: Yes. The columns are supplied empty (no resin) and are compatible with common IEX, affinity (Ni-NTA, Protein A/G, GST, etc.), and size-exclusion media.
Storage
Room temperature
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.

