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Empty columns with cap & stopper—reliable small-scale purification
$78.00
Sku: C006197-P
Description
The 20 mL Gravity Columns (total volume 32 mL) are economical, disposable tools designed for small-scale gravity flow protein and antibody purification, buffer exchange, and desalting. Supplied empty (no resin included), they feature leak-proof screw caps, durable polyethylene frits, and clear graduations—delivering flexibility, reliability, and cost-effective performance. Fully compatible with LabMate-style racks, these columns provide researchers with a convenient solution for rapid sample cleanup prior to SDS-PAGE, HPLC, or mass spectrometry.
Specification
| Package | 5 PCS/25/PK |
| Full name | Gravity Columns, 20 ml |
| Applications | small-scale protein and antibody purification, buffer exchange, desalting, and rapid sample cleanup before downstream analysis such as SDS-PAGE, HPLC, or mass spectrometry. |
| Summary | The 20 ml Columns have an internal volume of 32 ml and is designed for small scale gravity flow purifications. The resin bed volume is 20 ml. Supplied with screw caps and stoppers. |
| Features | Total Volume: 32mL；Resin Volume: 20mL；Reservoir Volume: 12mL；Graduated Closure: Plastic Stopper；Cap: Screw cap；Frit: 3mm ~30μm hydrophobic polyethylene |
| Composition | Columns, matching cap, frits, and rubber stoppers. |
| Total Volume | 32 ml |
| Resin Volume | 20 ml |
| Reservoir Volume | 12 ml |
| Closure | Plastic Stopper |
| Cap | Screw cap |
| Frit | 3 mm ~30 μm hydrophobic Polyethylene. |
| Component | Columns, matching cap, frits, and rubber stoppers. |
Gravity Columns, 20 mL – FAQ
Q: What are they used for?
A: Perfect for small-scale protein and antibody purification, buffer exchange, and quick sample cleanup before downstream analysis.
Q: What samples can I load?
A: Typically cell lysates, fermentation supernatants, or antibody-containing culture media.
Q: What quality matters most?
A: Leak-proof screw caps, stable frits for uniform flow, and clean, transparent columns ensure reliable and reproducible results.
Q: Any common challenges?
A: Flow control and recovery can be tricky, but our well-designed frits and column geometry minimize dead volume and sample loss.
Q: Why choose these columns?
A: They’re clean, consistent, and cost-effective—with multiple pack sizes (5 pcs / 25 pcs) for flexible lab use.
Q: Do these columns fit LabMate-style racks?
A: Yes—our 20 mL gravity columns fit most LabMate-style and universal chromatography racks designed for ~18 mm wells. If your rack uses special-size wells, please check the well diameter before purchase.
Q: Can I collect into 15 mL or 50 mL tubes?
A: Yes. The outlet geometry allows stable positioning over both 15 mL and 50 mL conical tubes.
Q: Are these compatible with ion-exchange/affinity/gel-filtration resins?
A: Yes. The columns are supplied empty (no resin) and are compatible with common IEX, affinity (Ni-NTA, Protein A/G, GST, etc.), and size-exclusion media.
Storage
Room temperature
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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