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Description
This product includes an enhanced luminol substrate and a stable peroxide system to detect femtogram amounts of antigen and is suitable for chemiluminescence by conventional Western Blot. This product contains an enhanced luminol substrate and a stable peroxide system for detecting femtogram amounts of antigen and is suitable for chemiluminescent detection in conventional Western Blot.
Features
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Stability:
24-hour working solution stability; 1-year kit stability at room temperature.
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High Cost Performance:
Compared with other chemiluminescent substrates of the same grade, the cost performance is quite high.
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High Sensitivity:
Quickly detect proteins as low as femtogram amounts. Its performance is significantly better than other high-end brand products of the same level.
Ultra-high sensitivity at real femtogram level
The 1 mg/mL actin-HRP antibody was serially diluted to obtain concentrations of 1000, 500, 250, 125, and 62.5 fg/μL. 1μL of each dilution was spotted onto an NC membrane. After spotting, the membrane was developed with YamayBio VQ4726 for 5 minutes.
YamayBio VQ4726 offers higher femtogram-level sensitivity than Thermo 37071.
Lysate: A431 whole cell lysates MW: GAPDH-36kD Primary antibody: Anti-GAPDH, dilution at 1:5000 Secondary antibody: Goat anti-Mouse IgG(H+L),HRP conjugated at 1:5000 dilution Exposure duration: 10 seconds.
Specifications
| Product Type | Substrate |
| Detection Method | Chemiluminescence |
| Form | Liquid |
| Application | Western Blot |
| Quantity | 100 mL / 500 mL |
| Sensitivity | Femtogram |
| Membrane Compatibility | Nitrocellulose, PVDF |
Storage
Transported at room temperature, stored at 4°C away from light, shelf life is 12 months.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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