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Single-strand oligo DNA probe with a 6-FAM label at the 5' end and a BHQ label at the 3' end.
Description
Cas12 ssDNA reporter is a single-strand oligo DNA probe with a 6-FAM (Fluorescence) label at the 5' end and a BHQ (quencher) label at the 3' end. It is designed to be used in CRISPR-Cas12 activity assays. When combined with guide RNA and target DNA, Cas12a, Cas12b, and Cas12f enzymes exhibit activated trans-cleavage activity against ssDNA. Upon cleavage, the quenched fluorescence of the probe recovers, enabling enzyme activity analysis and target detection.
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