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Specification
| Full name | AMV Reverse Transcriptase |
| Applications | 1. First-strand cDNA synthesis for RT-PCR and real-time RT-PCR; 2. It can increase the reaction temperature and reduce the influence of secondary structure on reverse transcription; 3. Synthesis of cDNA for cloning and expression research; 4. Synthetic labeled cDNA probe; 5. Primer extension method for RNA analysis. |
| Summary | This product is a recombinant avian myeloblastoma reverse transcriptase expressed by Escherichia coli. AMV RT is a heterodimer consisting of two distinct subunits (alpha 65 KDa and beta 94 KDa subunits). AMV RT has a variety of activities, including RNA and DNA-dependent DNA polymerase activity, DNA-RNA unwinding activity, sequence-specific Mn2+-dependent endonuclease activity, and RNase H (45~60°C) retention activity, so it can reverse transcribe RNA containing a large number of secondary complex structures. |
| Features | The product uses all imported raw materials, the properties are more stable, the active temperature is 37~58 °C, the reverse transcription temperature can reach 60 °C, and the full-length first-strand cDNA can be efficiently prepared, up to 13kb. |
| Composition | AMV RT (10 U/ul) and 5X AMV RT Buffer |
| Reaction Buffer | 5X AMV RT buffer |
| Unit Definition | One unit is defined as the amount ofenzyme required to catalyze the transfer of 1 nmol of Deoxynucleotidein to acid-precipitable material in 10 minutes at 37°C. |
| Thermal Inactivation | Heat at 85 °C for 5 min to lose activity. |
| Concentration | 10 U/μl |
| Storage Buffer | 200 mM potassium phosphate (pH 7.2 ,4°C), 2 mM DTT, 0.2% (V/V) Triton X-100 and 50% (V/V) glycerol. |
| Applications |
1. First-strand cDNA synthesis for RT-PCR and real-time RT-PCR; 2. It can increase the reaction temperature and reduce the influence of secondary structure on reverse transcription; 3. Synthesis of cDNA for cloning and expression research; 4. Synthetic labeled cDNA probe; 5. Primer extension method for RNA analysis.
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| Component | AMV RT (10 U/ul) and 5X AMV RT Buffer |
Storage
-20°C
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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