Specification

Full name	BsmBI
Applications	It is commonly used in experiments such as Golden Gate assembly, rapid cloning, and preparation of a large number of linearized plasmids
Summary	BsmBI is the isoschizoid enzyme of the SpeedyCut series restriction enzyme Esp3I, but because the reaction buffer is optimized to maximize the activity of BsmBI endonuclease, it becomes an independent system Buffer, which cannot be shared with the general SpeedyOne Buffer, and the recombinant albumin component is added to the reaction buffer, which greatly improves the stability of the enzyme.
Features	Stability is better than that of isoschizozyme Esp3I for a large number of linearized plasmid preparations.
Composition	1ul BsmBI,1X BsmBI buffer (dedicated)
Recognition Site	5'…-CGTCTC(N)1↓…-3' 3'…-GCAGAG(N)5↑…-5'
Reaction Buffer	1X BsmBI buffer (dedicated)
Recommended Reaction Temperature	55°C
Unit Definition	One unit of activity refers to the amount of enzyme required to completely digest 1 μg of λDNA in a 5 μl reaction system at 5℃ for 1 hour.
Methylation Effects	Dam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Sequences overlap completely, cleavage blocked; EcoKI: Methylation does not affect; EcoBI: Sequences may overlap and cleavage may be blocked.
Thermal Inactivation	Heat at 8°C for 2 min to lose activity

Storage

-20°C