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Description

The TC1000-G Gradient Thermal Cycler is designed for routine PCR amplification and gradient optimization experiments in molecular biology laboratories. With its precise temperature control system and programmable gradient function, it enables researchers to quickly determine optimal annealing conditions for different primer sets.

Unlike real-time PCR (qPCR) systems, the TC1000-G is a conventional endpoint PCR thermal cycler and does not support fluorescence detection. It is optimized for DNA amplification, cloning, genotyping, and standard PCR workflow development.

Note: The TC1000-G is a standard end-point PCR thermal cycler for conventional PCR amplification. It is not a real-time / quantitative PCR (qPCR) instrument and does not perform fluorescence detection. For real-time PCR applications, please refer to our qPCR systems.

Specifications

SKU 5064102300
Sample Capacity 96×0.2 mL PCR tube | 12×0.2 mL PCR-8 strips | 0.2 mL 96-well PCR microplate
Heating Temperature Range 4–105 °C
Lid Temperature Range 30–110 °C
Temperature Display Accuracy ±0.1 °C
Temperature Control Accuracy ±0.3 °C @55 °C
Temperature Uniformity @55 °C <0.3 °C @55 °C
Max. Heating/Cooling Rate 5 °C/sec
Gradient Temperature Setting Range 30–99 °C
Gradient Range 1–42 °C (turn off gradient at input 0)
Adapter Block Material Aluminum
Display 7" LCD (800×480)
Input Touch panel
User Defined File System Max. 30 segments, 99 cycles; storage for over 200 files
Power Off Protection Yes
Voltage / Frequency 100–120 V / 200–240 V, 50/60 Hz
Dimensions (W×D×H) 280 × 370 × 250 mm
Weight 11 kg

 

Features

  • Precise gradient PCR thermal cycler for PCR optimization and DNA amplification workflows

  • 4–105°C wide temperature control range with high stability and repeatability

  • Fast heating and cooling rate up to 5°C/sec for efficient cycling

  • 7-inch LCD touchscreen (800×480) for intuitive programming and operation

  • High-performance DSP control system ensures stable and accurate thermal cycling

  • Uniform aluminum block design ensures consistent temperature distribution across wells

  • Supports multi-segment programs with large storage capacity (>200 programs)

  • Gradient function enables rapid annealing temperature optimization

Applications

  • Standard PCR amplification of DNA/RNA targets

  • Gradient PCR for annealing temperature optimization

  • Gene cloning and plasmid construction

  • Genotyping and mutation detection

  • Microbial identification and strain verification

  • Routine molecular biology research workflows

  • Teaching and academic laboratory PCR training

  • Protocol development and optimization experiments

What’s Included

  • TC1000-G Gradient Thermal Cycler main unit

  • Standard 96-well aluminum block (pre-installed)

  • Power cable

  • User manual

Also Purchased / Recommended Models

The TC1000-G gradient thermal cycler is commonly selected alongside higher-capacity and advanced control models for different PCR workflow needs. Below is a comparison to help you choose the most suitable system for your laboratory.

TC1000-G vs TC2000-G1 Gradient PCR Thermal Cycler

Feature TC1000-G TC2000-G1
Price $4,055 $4,428
Display 7" LCD (800×480) 10.1" LCD (1280×800)
Block Heating Range 4–105 °C 0–105 °C
Temperature Control Accuracy ±0.3 °C ±0.1 °C
Temperature Uniformity @55 °C <0.3 °C ±0.2 °C
Gradient Setting Range 30–99 °C 30–105 °C
Gradient Range 1–42 °C 1–42 °C
Max Cycles per Program 99 199
Channels Single block Single / dual / triple channel
Sample Capacity 96 × 0.2 mL 96 × 0.2 mL
Best For Cost-effective routine gradient PCR Higher precision, multi-program / multi-channel needs

 

Which Model Should You Choose?

  • Choose TC1000-G if you need a compact, cost-efficient gradient PCR system for routine molecular biology experiments.

  • Choose TC2000-G1 if your workflow requires higher throughput, multi-program execution, or advanced operational control.

Recommended Workflow Pairings

  • PCR Tubes & 96-well Plates (0.2 mL formats)

  • qPCR / Real-Time PCR Systems (for fluorescence-based quantification workflows)

  • DNA Polymerase & PCR Master Mix

  • Gel Electrophoresis Systems for PCR product analysis

Note: TC1000-G and TC2000-G1 are conventional PCR thermal cyclers and are not suitable for real-time fluorescence detection (qPCR).

Frequently Asked Questions

Is the TC1000-G a real-time PCR (qPCR) system?

No. TC1000-G is a standard endpoint PCR thermal cycler and does not include fluorescence detection for real-time quantification.

What is gradient PCR used for?

Gradient PCR allows users to test multiple annealing temperatures in a single run, helping optimize primer binding conditions and improve amplification efficiency.

What is the difference between gradient PCR and normal PCR?

Normal PCR uses a single annealing temperature, while gradient PCR runs multiple temperatures across the block to identify optimal reaction conditions.

What sample formats are supported?

It supports 0.2 mL PCR tubes, 8-strip tubes, and standard 96-well PCR plates.

Can this instrument be used for RNA experiments?

Yes, but only after reverse transcription (RT-PCR workflow). It does not directly detect RNA; it performs thermal cycling for amplification steps.

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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