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Description
The TC2000-G1 Gradient Thermal Cycler is a high-performance PCR system designed for advanced molecular biology workflows requiring precise temperature control, fast cycling performance, and flexible gradient optimization. It is widely used for DNA amplification, gene cloning, genotyping, and protocol development in research laboratories and diagnostic environments.
With its upgraded thermal control system and expanded program capacity, the TC2000-G1 enables researchers to efficiently optimize annealing conditions through gradient PCR while maintaining high repeatability and temperature uniformity across the entire block.
Note: The TC2000-G1 is a conventional endpoint PCR thermal cycler. It does not support fluorescence detection and is not a real-time PCR (qPCR) instrument. It is intended for standard PCR amplification and gradient optimization workflows only.
Specifications
| SKU | 5034102400 |
| Sample Capacity | 96 × 0.2 mL PCR tubes | 12 × PCR-8 strips | 1 × 0.2 mL 96-well PCR plate |
| Heating Temperature Range | 0–105 ℃ |
| Lid Temperature Range | 30–115 ℃ |
| Temperature Display Accuracy | 0.1 ℃ |
| Temperature Control Accuracy | ±0.1 ℃ |
| Temperature Uniformity @55 ℃ | ±0.2 ℃ |
| Max. Heating / Cooling Rate | 5 ℃ / sec |
| Gradient Temperature Setting Range | 30–105 ℃ |
| Gradient Range | 1–42 ℃ (turn off gradient at input 0) |
| Adapter Block Material | Aluminum |
| Display | 10.1-inch LCD (1280 × 800) |
| Input | Touch panel |
| User Defined File System | Up to 30 steps and 199 cycles per program |
| Power Off Protection | Yes |
| Voltage / Frequency | 100–240 V, 50/60 Hz, 600 W |
| Dimensions (W×D×H) | 310 × 422 × 264 mm |
| Weight | 11 kg |
Features
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High-precision gradient PCR system for annealing temperature optimization and DNA amplification workflows
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Wide temperature range (0–105°C) with high stability and reproducibility
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Fast thermal cycling performance up to 5°C/sec for improved experimental efficiency
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10.1-inch high-resolution LCD touchscreen (1280×800) for intuitive operation
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Advanced thermal control system ensures precise temperature regulation and uniformity
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High-quality aluminum block design provides consistent heat distribution across all wells
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Supports up to 199 cycles and 30-step programming for complex experimental protocols
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Gradient function enables rapid optimization of PCR annealing conditions
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Real-time program status display with secure user account management options
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Power-off protection with automatic recovery of interrupted PCR programs
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USB interface for data transfer, protocol storage, and software updates
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Compatible with 0.2 mL PCR tubes, 8-strip tubes, and 96-well PCR plates (no skirt / semi-skirt)
Applications
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Standard PCR amplification of DNA templates
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Gradient PCR for annealing temperature optimization
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Gene cloning and recombinant DNA construction
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Genotyping and mutation detection
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Microbial identification and strain verification
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Gene expression and molecular biology research
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Protocol development and PCR workflow optimization
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Academic teaching and laboratory training
What’s Included
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TC2000-G1 Gradient Thermal Cycler main unit
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96-well aluminum block (pre-installed)
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Power cable
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User manual
Also Purchased / Recommended Models
The TC1000-G gradient thermal cycler is commonly selected alongside higher-capacity and advanced control models for different PCR workflow needs. Below is a comparison to help you choose the most suitable system for your laboratory.
TC1000-G vs TC2000-G1 Gradient PCR Thermal Cycler
| Feature | TC1000-G | TC2000-G1 |
| Price | $4,055 | $4,428 |
| Display | 7" LCD (800×480) | 10.1" LCD (1280×800) |
| Block Heating Range | 4–105 °C | 0–105 °C |
| Temperature Control Accuracy | ±0.3 °C | ±0.1 °C |
| Temperature Uniformity @55 °C | <0.3 °C | ±0.2 °C |
| Gradient Setting Range | 30–99 °C | 30–105 °C |
| Gradient Range | 1–42 °C | 1–42 °C |
| Max Cycles per Program | 99 | 199 |
| Channels | Single block | Single / dual / triple channel |
| Sample Capacity | 96 × 0.2 mL | 96 × 0.2 mL |
| Best For | Cost-effective routine gradient PCR | Higher precision, multi-program / multi-channel needs |
Which Model Should You Choose?
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Choose TC1000-G if you need a compact, cost-efficient gradient PCR system for routine molecular biology experiments.
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Choose TC2000-G1 if your workflow requires higher throughput, multi-program execution, or advanced operational control.
Recommended Workflow Pairings
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PCR Tubes & 96-well PCR Plates (0.2 mL formats, no skirt / semi-skirt compatible)
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PCR Reagents (Taq DNA Polymerase, High-Fidelity DNA Polymerase, PCR Master Mix)
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DNA Extraction & Purification Kits for upstream template preparation
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Gel Electrophoresis Systems for PCR product validation and analysis
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DNA Ladders & Loading Buffers for fragment size confirmation
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qPCR / Real-Time PCR Systems (for downstream fluorescence-based quantification workflows)
Note: The TC2000-G1 is a conventional endpoint PCR thermal cycler and does not support real-time fluorescence detection (qPCR). It is intended for standard PCR amplification and gradient optimization workflows only.
Frequently Asked Questions
Is the TC2000-G1 a real-time PCR (qPCR) instrument?
No. The TC2000-G1 is a conventional endpoint PCR thermal cycler and does not include fluorescence detection for quantitative PCR.
What is gradient PCR used for?
Gradient PCR allows multiple annealing temperatures to be tested in a single run, helping quickly identify optimal amplification conditions.
What makes TC2000-G1 different from standard PCR cyclers?
It offers higher temperature accuracy (±0.1°C), improved uniformity, a larger touchscreen interface, and support for multi-step, high-cycle programming up to 199 cycles.
What sample formats are supported?
It supports 0.2 mL PCR tubes, 8-strip tubes, and 96-well PCR plates (no skirt and semi-skirt types).
Does it support complex PCR programs?
Yes. It supports up to 30 program steps and 199 cycles, suitable for advanced PCR workflows and protocol optimization.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.

