Residual Host Cell DNA Sample Preparation Kit (For manual and automated operation) SHENTEK™

For Research Use Only (RUO)

$1,010.00

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Sku: 1104191
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⚡ Detection Method
qPCR
🕒 Assay Time
Refer to manual
🎯 Storage Temperature
2-8 °C or -20 °C (stable for 24 months)

Product Description

SHENTEK® Residual Host Cell DNA Sample Preparation Kit is a magnetic particle separation-based sample preparation kit designed for efficient and reproducible extraction of trace residual host cell DNA from a wide range of biological products and complex matrices across different manufacturing steps. The kit is compatible with manual workflows or automated extraction using the rHCDpurify instrument and is intended for use prior to downstream host cell DNA quantitation and size analysis (e.g., with SHENTEK host cell DNA quantitation and size analysis kits). The kit includes reagents for enzymatic protein digestion (Proteinase K), binding, washing and elution of nucleic acids and is optimized for multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 & AcNPV, Hi5 & AcNPV, plasmid, SV40LTA & EIA. Product No.: 1104191. Reagents for 100 extractions. For Research Use Only (RUO).

Technical Specifications

Parameter Details
Intended use / application Extraction of residual host cell DNA from biological products and complex sample matrices prior to quantitation and size analysis
Reactions per kit Reagents for 100 extractions
Sample input volume (example protocol) 100 µL sample per extraction (protocol example uses 100 µL sample)
Proteinase K digestion Prepare Proteinase K digestion solution per 100 µL sample: for sample protein 0–100 mg/mL add 10 µL Proteinase K to 100 µL Proteinase K Buffer; for 100–200 mg/mL add 20 µL Proteinase K to 100 µL Proteinase K Buffer. Incubate at 55 °C for 60 min.
Binding buffer per sample 200 µL Binding solution + 9 µL Glycogen + 0.2 µL Yeast tRNA (omit Yeast tRNA for E.coli and yeast DNA extractions)
Magnetic particles 10 µL magnetic particles per sample; vortex to resuspend before use; recommended storage 2-8 °C
Isopropanol Add 200 µL isopropanol per sample during binding step
Wash steps Wash A: 700 µL Wash buffer A; Wash B: 700 µL Wash buffer B; remove residual liquid and air-dry pellet 30 s–3 min depending on environment
Elution 50–100 µL pre-warmed (70 °C) Elution buffer; incubate at 70 °C for 7 min with intermittent mixing; centrifuge and separate on magnetic stand prior to transfer
Critical temperatures and times Proteinase K digestion: 55 °C for 60 min; Elution: 70 °C for 7 min; vortex binding for 5 min; resuspend magnetic particles before use
Storage stability Kit components stored at indicated temperatures can be kept up to 24 months (check labels for expiration dates)
pH and ionic requirements Sample pH should be adjusted to neutral (pH 6.0–8.0). Adjust ionic concentration with 5M NaCl if needed.
Compatibility Compatible with manual workflows and with the rHCDpurify instrument; downstream use with SHENTEK host cell DNA quantitation and size analysis kits and real-time PCR systems
Product number 1104191

Features

  • Magnetic Particle Separation: Magnetic particle-based extraction provides efficient capture and purification of trace residual DNA from complex matrices.
  • Broad Host Cell Compatibility: Validated for multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 & AcNPV, Hi5 & AcNPV, plasmid, SV40LTA & EIA.
  • High Recovery from Complex Matrices: Optimized binding, wash and elution steps including Proteinase K digestion to improve recovery from protein- or matrix-rich samples.
  • Manual and Automated Workflows: Can be used in manual bench workflows or with the rHCDpurify automated instrument for higher throughput and consistency.
  • Validated Workflow Inputs: Includes reagent recipes and stepwise protocol (binding buffer composition, enzyme digestion volumes, wash volumes and temperatures) for reproducible extraction.
  • Ready for Downstream qPCR or Size Analysis: Eluted DNA is suitable for downstream host cell DNA quantitation and size analysis workflows.

Applications

  • Extraction of residual host cell DNA from biologics and bioprocess samples
  • Sample preparation prior to host cell DNA quantitation (e.g., qPCR)
  • Sample preparation prior to DNA size/fragment analysis
  • Quality control and in-process monitoring of upstream and downstream manufacturing steps
  • Recovery of trace DNA from complex matrices (formulations, buffers, cell lysates, clarified harvests)

Kit Contents

Wash buffer A (NND014)Room temperature (add 40 mL anhydrous ethanol before first use)
Binding solution (NND016)Room temperature
Elution buffer (NND018)Room temperature
Dilution buffer (NND021)Room temperature
Proteinase K Buffer (NND026)Room temperature (if cloudy heat at 37 °C to dissolve)
Magnetic particles (NND030) 750 µL × 22-8 °C
Proteinase K (NND023) 500 µL × 2-20 °C
Glycogen (NND035) 500 µL × 2-20 °C
Yeast tRNA (NND037) 50 µL × 1-20 °C (omit for E.coli and yeast DNA extractions)
* Total: 100 extractions | Method: Magnetic particle-based DNA extraction

Attention

• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, eyewear, lab coat).

• Add 40 mL anhydrous ethanol to Wash buffer A before first use and prepare Wash buffer B as 70% ethanol.

• Adjust sample pH to neutral (pH 6.0–8.0) with 1M HCl or 1M NaOH prior to extraction.

• Prepare Proteinase K digestion solution according to sample protein concentration; digestion at 55 °C for 60 min is critical for DNA recovery.

• Do not add Yeast tRNA when extracting E. coli or yeast DNA.

• Resuspend magnetic particles thoroughly immediately before use; avoid over-drying the pellet (30 s–3 min) to ensure efficient elution.

• Perform centrifugation immediately after vortexing to collect liquid and particles from tube walls and caps.

• Perform downstream assays the same day after nucleic acid extraction to ensure accurate results.

• Store magnetic particles at 2–8 °C; storing at -20 °C may reduce performance.

Quality Management & Certifications

Quality System

Download QMS

QMS (ISO, GMP)

Download Certificate

Quality Advantages

View Report

Quality Control Process

Download Process

📂 Technical Resources & Downloads

PDF Product Manual ↓ PDF MSDS / SDS ↓

Frequently Asked Questions (FAQ)

Q1: What sample types are compatible with this kit?
The kit is compatible with a wide range of sample matrices from multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 & AcNPV, Hi5 & AcNPV, plasmid, SV40LTA & EIA. It is intended for biological products at various manufacturing steps.
Q2: How much sample volume is used in the protocol?
The provided example protocol uses 100 µL sample per extraction. Adjustments may be made but follow recommended binding and enzyme volumes for optimal recovery.
Q3: How should I prepare Proteinase K digestion solution?
For each 100 µL sample: if sample protein is 0–100 mg/mL add 10 µL Proteinase K to 100 µL Proteinase K Buffer; if sample protein is 100–200 mg/mL add 20 µL Proteinase K to 100 µL Proteinase K Buffer. Incubate at 55 °C for 60 min.
Q4: Can I use this kit with automated instruments?
Yes. The kit is compatible with manual workflows and automated extraction using the rHCDpurify instrument; ensure instrument settings follow the recommended incubation times and mixing steps.

Research Use Only

✔

Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.

✔

Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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