Human Rhinovirus 54 Probe qRT-PCR Kit

Description

Human Rhinovirus 54 Probe qRT-PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

• High sensitivity: analytical LOD as low as 100 copies/reaction

• Excellent specificity: no cross-reactivity with DNA from other pathogens

• Includes amplification positive control for result verification

• Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.

• Suitable for both qualitative and quantitative applications

• For quantitative use: the linear range covers a 100,000-fold concentration difference.

• Sufficient for 50 reactions (20 μL each)

• For Research Use Only (RUO)

Kit Components

Application Areas

• Clinical diagnostics and patient management: Rapid, high-throughput qRT-PCR screening of respiratory specimens for precise HRV54 detection, enabling differential diagnosis and tailored treatment strategies.
• Epidemiological surveillance and outbreak response: Sensitive quantification of HRV54 viral load in community and hospital settings to track transmission dynamics and support public health interventions.
• Pharmaceutical research and vaccine development: Robust HRV54 quantification for antiviral efficacy testing, vaccine candidate evaluation, and molecular pathogenesis studies in preclinical and translational workflows.

Storage & Shipping

• Store at - 20 °C

• The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
  

Documents

Human Rhinovirus 54 Probe qRT-PCR Kit User Manual

MSDS