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Rapid and Sensitive qRT-PCR Kit for Precise Quantification of Escherichia coli Total RNA in Environmental and Clinical Samples – 50 Reactions
$641.00
Sku: 15-175R
Categories:
Pathogen Detection Kits
, qPCR Kit
, qRT-PCR Kit
Description
Escherichia coli Total RNA Residual Probe Method qRT-PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.
Key Features
One-tube qPCR format minimizes carry-over contamination risk
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High sensitivity: analytical LOD as low as 100 copies/reaction
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Excellent specificity: no cross-reactivity with DNA from other pathogens
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Includes amplification positive control for result verification
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Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.
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Suitable for both qualitative and quantitative applications
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For quantitative use: the linear range covers a 100,000-fold concentration difference.
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Sufficient for 50 reactions (20 μL each)
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For Research Use Only (RUO)
Kit Components
| Component Name | Product Number. | Size | Vial | Lid Color |
| 2×Probe qPCR MasterMix | 981201 | 0.5 mL | 0.5 mL Vial | Natural |
| Template Dilution Buffer | 180701 | 1 mL | 1.5 mL Vial | Green |
| Primer-Probe Powder | yp15-175R | 50 T | 0.5 mL Brown Vial | Brown |
| Amplification Positive Control (1E7 copies/μL) |
pc175R | 50 μL | 0.5 mL Vial | Yellow |
| Ultrapure water | 210806 | 1 mL | 1.5 mL Vial | Blue |
| Manual | sc15-175R | 1 Set | NA | NA |
Application Areas
- Seafood Processing & Quality Control: Rapid qRT-PCR–based screening of Escherichia coli residual RNA in shellfish and marine products to ensure compliance with HACCP, FDA/EFSA regulations and minimize product recalls.
- Aquaculture Health Monitoring: High-sensitivity detection of E. coli total RNA in abalone, shrimp and fish farms for real-time disease surveillance, pathogen risk assessment and optimized farm management.
- Marine Environmental Surveillance: Quantitative tracking of E. coli residual RNA in seawater, sediment and biofilms to support ecological risk assessments, water quality monitoring and coastal ecosystem protection.
Storage & Shipping
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Store at - 20 °C
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The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
Documents
Escherichia coli Total RNA Residual Probe Method qRT-PCR Kit User Manual
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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