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Description
The kit employs a solid-phase Enzyme-linked Immunosorbent Assay (ELISA) with a double-antibody sandwich technique to detect residual CHO K1 host cell proteins in the samples. Polyclonal antibody specific to CHO K1 HCPs was employed in the assay to capture any remaining HCPs in the samples. Both the Calibration standards (or test samples) and the HRP (Horseradish Peroxidase) labeled anti-CHO K1 HCPs antibody were simultaneously added to the microtiter plate, which coated with the affinity purified capture antibody and followed by incubation and washing. Then TMB (3,3',5,5' -tetramethylbenzidine) substrate was added into the reaction, HRP catalyzed the oxidation of TMB by H2O2 to produce a blue product (maximum absorption peak at 655 nm). The stop solution is added to terminate the enzymatic reaction, resulting in a yellow color product (maximum absorption peak at 450 nm). The absorbance values at 450 nm wavelength were positively correlated with the HCPs concentration in the Calibration standards and the samples. The concentration of CHO K1 HCPs in the samples can be calculated using the dose-response curve.
| Components | Shipping Condition | Unit Size | Detection Method |
| CHO K1 HCP Calibration Standard | 2-8℃ | 96 Tests | Sandwich ELISA |
| Anti-CHO K1:HRP (250×) | |||
| Anti-CHO K1 HCP Microtiter Strips | |||
| Wash Buffer Concentrate (10×) | |||
| TMB Substrate | |||
| BSA | |||
| Stop Solution | |||
| Sealing Film |
Specifications
| Linearity & Range | 3-100 ng/mL, R2>0.990 |
| Accuracy | Recovery Rate= 93%-110% |
| LLOQ | 3 ng/mL |
| Antibody Coverage (2D-WB) | 80% |
| Specificity | No cross-reactivity with MDCK, Vero, HEK293T, E.coli, P.pastoris and SF9 cells. |
| Robustness | Freeze-thaw stability: CHO-K1 HCP Calibration standards and Anti-CHO:HRP (250×) can be used no more than 3 cycles |
| Storage stability: The HCP Calibration standards and Anti-CHO:HRP (250×) can store at ≤-18℃ for 24 months |
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When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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