For Research Use Only (RUO)

$2,575.00

FREE
SHIPPING

100% MONEY
BACK GUARANTEE

ONLINE
SUPPORT 24/7

Description

SHENTEK® Residual SV40LTA & E1A DNA Quantitation Kit (2G) is used to quantitate residual SV40LTA & E1A host cell DNA, such as HEK293T cell. This sv40 large t antigen kit uses duplex real-time PCR technique to performs rapid, specific, and reliable quantitation assay at the level of 101 copies/μL. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191)

Introduction

Components Shipping Condition Unit Size Detection Method
SV40LTA & E1A linear DNA Control -20℃ 100 Reactions Probe-basedq PCR
SV40LTA & E1A non-linear DNA Control
qPCR Reaction Buffer -20℃, protect from light
SV40LTA & E1A Primer & Probe MIX
IPC MIX
DNA Dilution Buffer (DDB) -20℃

 

Specification

Range SV40LTA: 4.67x101-4.67x106 pg/μL, R2=0.99974, E=97.5%
E1A: 4.97x101-4.97x106 pg/μL, R2=0.99946, E=98.3%
Accuracy

SV40LTA: Recovery = 105.2%-110.1%, CV<30%
E1A: Recovery = 115.8%-124.5%, CV<30%
LOQ
SV40LTA: 9.34 copies/μL
E1A: 49.7 copies/μL
LOD
SV40LTA: 0.934 copies/μL
E1A: 0.497 copies/μL
Precision
SV40LTA: 1.7%-9.0% (<30%)
E1A: 1.9%-9.4%(<30%)
Robustness
Freeze-thaw stability over 5 cycles
Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments

 

Documents

User Guide

Related Products

SHENTEK™ Residual Host Cell DNA Sample Preparation Kit

Research Use Only

For Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.

Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

Terms and Conditions