For Research Use Only (RUO)

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Description

SHENTEK™ Residual E. coli RNA Quantitation Kit (2G) is used for quantitation of host cell RNA from E. coli in a variety of biopharmaceutical products. This kit employs duplex reverse transcription quantitative PCR technique to performs a rapid, specific, and reliable quantitative detection of residual E. coli RNA .The target gene (FAM) can be determined at the femtogram (fg) level, whereas IPC-Internal Positive Control (VIC) is also included in the E. coli RNA Primer&Probe MIX to evaluate the performance of each PCR reaction. 

Introduction

Components Shipping Condition Unit Size Product Line Detection Method
E.coli RNA Control -20℃ 100 Reactions SHENTEK™ Probe-based qPCR
One Step qPCR Buffer -20℃, protect from light      
One Step Enzyme MIX -20℃, protect from light      
E.coli RNA Primer&Probe MIX (Incl IPC) -20℃, protect from light      
RNase-Free H2O -20℃      

 

Specification

Range 2x10-2-2x102pg/μL, R2 ≥0.990E=83.3%~110%
Accuracy 64.1%-97.8%, CV<30%
LOQ 0.002 pg/μL
Precision 10.7%-13.1% (<30%)
Robustness Freeze-thaw stability over 5 cycles
Instrument suitability not limited to Thermo, Biorad, HZSKBio qPCR equipments

 

Documents

User Guide

Related Products

SHENTEK™ Residual Host Cell DNA Sample Preparation Kit

Research Use Only

For Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.

Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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