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For Research Use Only (RUO)
Description
The pcr amplification kits are used for quantitation of residual E. coli host cell DNA in a variety of biopharmaceutical products.
SHENTEK® Residual E.coli DNA Quantitation Kit is used for quantitation of residual E. coli host cell DNA in a variety of biopharmaceutical products. This kit uses quantitative PCR to perform rapid and specific quantitation of residual DNA at the femtogram level. The assay is accurate and reliable across a broad range of sample types, from in-process samples to final products. The kit comes with E. coli Control DNA, which is traceable to a primary DNA reference standard.
For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. SK030203D100)
Introduction
| Components | Shipping Condition | Unit Size | Detection Method |
| E. coli DNA Control | -20℃ | 100 Reactions | Probe-based qPCR |
| E. coli qPCR MIX | -20℃, protect from light | ||
| DNA Dilution Buffer (DDB) | -20℃ |
Specification
| Range | 3x10-2-3x102 pg/μL, R2=0.99968, E=97.5% |
| Accuracy | Recovery = 81.2%-107.4% , CV<30% |
| LOQ | 0.03 pg/μL |
| LOD | 0.003 pg/μL |
| Precision | 2.8%-17.9% (<30%) |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments |
Documents
Related Products
Residual Host Cell DNA Sample Preparation Kit - SHENTEK®
Research Use Only
For Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.
Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.

