Vaccinium oxycoccus-Ingredient Probe qPCR Kit

Description

Vaccinium oxycoccus-Ingredient Probe qPCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

• High sensitivity: analytical LOD as low as 100 copies/reaction

• Excellent specificity: no cross-reactivity with DNA from other pathogens

• Includes amplification positive control for result verification

• Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.

• Suitable for both qualitative and quantitative applications

• For quantitative use: the linear range covers a 100,000-fold concentration difference.

• Sufficient for 50 reactions (20 μL each)

• For Research Use Only (RUO)

Kit Components

Application Areas

• Food Authentication & Quality Control: Quantitative detection of Vaccinium oxycoccus DNA in cranberry juices, extracts and dietary supplements to prevent adulteration, verify label claims and meet regulatory standards in the food & beverage and nutraceutical industries.
• Agricultural Research & Plant Breeding: Sensitive monitoring of Vaccinium oxycoccus genetic purity and expression in breeding programs, enabling molecular marker–guided selection, pathogen resistance studies and germplasm conservation for crop improvement.
• Environmental & Ecological Surveillance: High-throughput screening of wild and cultivated cranberry populations in wetlands, supporting biodiversity assessments, ecological monitoring and early disease detection in agricultural and conservation projects.

Storage & Shipping

• Store at - 20 °C

• The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
  

Documents

Vaccinium oxycoccus-Ingredient Probe qPCR Kit User Manual

MSDS