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Description
Rhaphiolepis bibas-Ingredient Probe qPCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.
Key Features
One-tube qPCR format minimizes carry-over contamination risk
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High sensitivity: analytical LOD as low as 100 copies/reaction
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Excellent specificity: no cross-reactivity with DNA from other pathogens
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Includes amplification positive control for result verification
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Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.
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Suitable for both qualitative and quantitative applications
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For quantitative use: the linear range covers a 100,000-fold concentration difference.
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Sufficient for 50 reactions (20 μL each)
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For Research Use Only (RUO)
Kit Components
| Component Name | Product Number. | Size | Vial | Lid Color |
| 2×Probe qPCR MasterMix | 981201 | 0.5 mL | 0.5 mL Vial | Natural |
| Template Dilution Buffer | 180701 | 1 mL | 1.5 mL Vial | Green |
| Primer-Probe Powder | yp15-221 | 50 T | 0.5 mL Brown Vial | Brown |
| Amplification Positive Control (1E7 copies/μL) |
pc221 | 50 μL | 0.5 mL Vial | Yellow |
| Ultrapure water | 210806 | 1 mL | 1.5 mL Vial | Blue |
| Manual | sc15-221 | 1 Set | NA | NA |
Application Areas
- Aquaculture Disease Surveillance: Employ the Probe qPCR Kit for high-sensitivity quantification of Rhaphiolepis bibas-Ingredient DNA in abalone, shrimp, and fish hatcheries to detect early-stage infections, support biosecurity programs, and optimize animal health management.
- Environmental DNA (eDNA) Monitoring: Leverage specific FAM-based qPCR assays to survey coastal waters, sediments, and aquaculture effluents for Rhaphiolepis bibas-Ingredient eDNA, facilitating ecosystem health assessments, biodiversity studies, and pollution impact analyses.
- Seafood Safety & Ingredient Authentication: Integrate the quantitative qPCR platform into quality control pipelines to authenticate Rhaphiolepis bibas-Ingredient presence in aquafeed additives, processed seafood, and marine-derived nutraceuticals, ensuring compliance with regulatory standards and traceability requirements.
Storage & Shipping
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Store at - 20 °C
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The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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