Poultry-Ingredient Probe PCR Kit

Description

Poultry-Ingredient Probe PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

• High sensitivity: analytical LOD as low as 100 copies/reaction

• Excellent specificity: no cross-reactivity with DNA from other pathogens

• Includes amplification positive control for result verification

• Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.

• Suitable for both qualitative and quantitative applications

• For quantitative use: the linear range covers a 100,000-fold concentration difference.

• Sufficient for 50 reactions (20 μL each)

• For Research Use Only (RUO)

Kit Components

Application Areas

• Aquaculture Feed Authentication & Quality Control: Real-time PCR detection of poultry DNA in marine feed formulations to ensure ingredient traceability, prevent cross-contamination, and meet regulatory standards in commercial aquaculture operations.
• Abalone Health Monitoring & Disease Surveillance: Quantitative screening of water and tissue samples for poultry-derived genetic markers, enabling early detection of zoonotic pathogens and supporting proactive health management in abalone farming.
• Coastal Ecosystem Monitoring & Pollution Assessment: High-sensitivity analysis of seawater and sediment for poultry DNA signatures, facilitating environmental impact studies, agricultural runoff tracking, and marine conservation research.

Storage & Shipping

• Store at - 20 °C

• The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
  

Documents

Poultry-Ingredient Probe PCR Kit User Manual

MSDS