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All-in-One qPCR Kit for Sensitive Detection of Lime Witches’ Broom Phytoplasma – 50 Reactions
$456.00
Sku: 15-2760
Description
Lime witches’ broom phytoplasma Probe Realtime PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.
Key Features
One-tube qPCR format minimizes carry-over contamination risk
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High sensitivity: analytical LOD as low as 100 copies/reaction
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Excellent specificity: no cross-reactivity with DNA from other pathogens
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Includes amplification positive control for result verification
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Compatible with various DNA purification methods
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Suitable for both qualitative and quantitative applications
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For quantitative use: the linear range covers a 100,000-fold concentration difference.
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Sufficient for 50 reactions (20 μL each)
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For Research Use Only (RUO)
Kit Components
| Component Name | Product Number. | Size | Vial | Lid Color |
| 2×Probe qPCR MasterMix | 981201 | 0.5 mL | 0.5 mL Vial | Natural |
| Template Dilution Buffer | 180701 | 1 mL | 1.5 mL Vial | Green |
| Primer-Probe Powder | yp15-2760 | 50 T | 0.5 mL Brown Vial | Brown |
| Amplification Positive Control (1E7 copies/μL) |
pc2760 | 50 μL | 0.5 mL Vial | Yellow |
| Ultrapure water | 210806 | 1 mL | 1.5 mL Vial | Blue |
| Manual | sc15-2760 | 1 Set | NA | NA |
Application Areas
- Sensitive early detection and quantification of Lime witches’ broom phytoplasma in citrus plant tissues (e.g., leaf midribs, bark/phloem, rootstocks) for research and nursery screening of propagation material — useful for identifying asymptomatic infections and monitoring pathogen load (For Research Use Only; not for clinical or regulatory diagnostics).
- High‑throughput surveillance in plant pathology and epidemiological studies, enabling large‑scale screening of field or greenhouse samples to support studies of disease incidence, seasonal dynamics, and vector–pathogen interactions.
- Quantitative monitoring in breeding, resistance evaluation, and control‑measure trials, allowing comparison of pathogen titers across cultivars, treatments, timepoints, and graft‑transmission experiments to inform research decisions.
Storage & Shipping
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Store at - 20 °C
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The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
FAQ
Q: Are Biofargo PCR and qPCR Kits compatible with my thermal cycler or real-time PCR instrument?
A: Yes. Biofargo PCR Kits and probe-based qPCR Kits are designed for broad compatibility with mainstream instruments.
PCR Kits can be used with standard thermal cyclers from leading brands.
Probe-based qPCR Kits are fully compatible with major real-time PCR platforms, including ABI, Bio-Rad, Roche, QuantStudio, Qiagen Rotor-Gene Q (5-plex), and Genesig Q16.
Our technical team is available to assist with instrument setup and run optimization to ensure the best results.
Q: Can this kit be used for digital droplet PCR (ddPCR)?
A: Yes.
Documents
Lime witches’ broom phytoplasma Probe Realtime PCR Kit User Manual
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
