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Highly Sensitive qPCR Kit for Rapid Haemophilus influenzae Detection in Clinical and Environmental Samples – 50 Reactions
$456.00
Sku: 15-11715
Categories:
Pathogen Detection Kits
, qPCR Kit
Description
Haemophilus influenzae V Probe Realtime PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.
Key Features
One-tube qPCR format minimizes carry-over contamination risk
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High sensitivity: analytical LOD as low as 100 copies/reaction
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Excellent specificity: no cross-reactivity with DNA from other pathogens
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Includes amplification positive control for result verification
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Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.
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Suitable for both qualitative and quantitative applications
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For quantitative use: the linear range covers a 100,000-fold concentration difference.
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Sufficient for 50 reactions (20 μL each)
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For Research Use Only (RUO)
Kit Components
| Component Name | Product Number. | Size | Vial | Lid Color |
| 2×Probe qPCR MasterMix | 981201 | 0.5 mL | 0.5 mL Vial | Natural |
| Template Dilution Buffer | 180701 | 1 mL | 1.5 mL Vial | Green |
| Primer-Probe Powder | yp15-11715 | 50 T | 0.5 mL Brown Vial | Brown |
| Amplification Positive Control (1E7 copies/μL) |
pc11715 | 50 μL | 0.5 mL Vial | Yellow |
| Ultrapure water | 210806 | 1 mL | 1.5 mL Vial | Blue |
| Manual | sc15-11715 | 1 Set | NA | NA |
Application Areas
- Aquaculture Disease Surveillance: High-throughput quantification of Haemophilus influenzae V in fish, shellfish and abalone farms for early outbreak detection, risk assessment and health management.
- Seafood Safety & Quality Control: Routine screening in processing plants, regulatory and contract testing laboratories to ensure compliance with microbial safety standards and prevent product recalls.
- Marine Ecology & Environmental Research: Sensitive monitoring of H. influenzae V dynamics in coastal and aquaculture ecosystems to support ecological impact studies, environmental risk assessments and biodiversity conservation.
Storage & Shipping
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Store at - 20 °C
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The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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