0 item(s)
View cart
You have no items in your shopping cart.
All-in-One qPCR Kit for Sensitive Detection of Anguina agrostis in Turfgrass – 50 Reactions
$456.00
Sku: 15-1130
Categories:
Pathogen Detection Kits
, qPCR Kit
Description
Anguina agrostis Probe Realtime PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.
Key Features
One-tube qPCR format minimizes carry-over contamination risk
-
High sensitivity: analytical LOD as low as 100 copies/reaction
-
Excellent specificity: no cross-reactivity with DNA from other pathogens
-
Includes amplification positive control for result verification
-
Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.
-
Suitable for both qualitative and quantitative applications
-
For quantitative use: the linear range covers a 100,000-fold concentration difference.
-
Sufficient for 50 reactions (20 μL each)
-
For Research Use Only (RUO)
Kit Components
| Component Name | Product Number. | Size | Vial | Lid Color |
| 2×Probe qPCR MasterMix | 981201 | 0.5 mL | 0.5 mL Vial | Natural |
| Template Dilution Buffer | 180701 | 1 mL | 1.5 mL Vial | Green |
| Primer-Probe Powder | yp15-1130 | 50 T | 0.5 mL Brown Vial | Brown |
| Amplification Positive Control (1E7 copies/μL) |
pc1130 | 50 μL | 0.5 mL Vial | Yellow |
| Ultrapure water | 210806 | 1 mL | 1.5 mL Vial | Blue |
| Manual | sc15-1130 | 1 Set | NA | NA |
Application Areas
- Aquaculture Biosecurity: Rapid, quantitative screening of Anguina agrostis in abalone, oyster and shrimp farms to protect stock health, prevent disease outbreaks and optimize production yields.
- Marine Pathogen Surveillance: High-sensitivity monitoring of nematode load in environmental and biodiversity studies for proactive ecosystem management and research-grade data accuracy.
- Seafood Safety & Quality Assurance: Routine PCR-based testing in seafood processing and regulatory laboratories to ensure compliance, assess contamination risks and uphold consumer safety standards.
Storage & Shipping
-
Store at - 20 °C
-
The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
No data
