Acidovorax konjaci Probe Realtime PCR Kit

Description

Acidovorax konjaci Probe Realtime PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

• High sensitivity: analytical LOD as low as 100 copies/reaction

• Excellent specificity: no cross-reactivity with DNA from other pathogens

• Includes amplification positive control for result verification

• Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.

• Suitable for both qualitative and quantitative applications

• For quantitative use: the linear range covers a 100,000-fold concentration difference.

• Sufficient for 50 reactions (20 μL each)

• For Research Use Only (RUO)

Kit Components

Application Areas

• Abalone and Shellfish Aquaculture Health Management: Rapid, sensitive qPCR-based detection and quantification of Acidovorax konjaci in water and tissue samples for early disease diagnosis, biosecurity enhancement, and mortality reduction in high‐value shellfish operations.
• Marine Pathogen Surveillance & Regulatory Compliance: High-throughput screening of environmental and hatchery samples to monitor A. konjaci prevalence, support risk assessment, and ensure adherence to aquaculture health standards and trade regulations.
• Seafood Safety and Ecosystem Monitoring: Quantitative tracking of A. konjaci contamination in seafood products and coastal environments, enabling quality control, traceability, and sustainable management of marine resources.

Storage & Shipping

• Store at - 20 °C

• The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
  

Documents

Acidovorax konjaci Probe Realtime PCR Kit User Manual

MSDS