Precast-Gel Hepes-Tris SDS-PAGE 4-20% 10Well

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Sku: P310062-10 PCS
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Description

Precast-GLgel Hepes SDS-PAGE 4–20% (10-Well) is a ready-to-use gradient gel designed for high-resolution protein electrophoresis. Using glass plate construction, it minimizes non-specific adsorption and produces sharper, clearer protein bands. This 4–20% HEPES-based gradient gel is pre-cast and supplied with running buffer, eliminating the need for hand-casting and greatly improving reproducibility.

The wide gradient range and stable HEPES buffer system make the gel especially suitable for separating large molecular weight proteins and membrane protein complexes, which often require gentle handling and high-resolution gradient gels.

Specification

Full name Precast-GLgel Hepes SDS-PAGE 4–20% 10-Well
Package 10 pcs
Components 10 pcs gel, 10 pcs SDS-PAGE running buffer
Gel size 8 × 7.4 × 0.15 cm
Plate size 9.8 × 8.4 × 0.41 cm
Wells 10 wells
Buffer system HEPES buffer system
Maximum sample loading volume 60 µL
Recommended sample loading volume 30 µL

 

Buffer

Buffer Hepes Running Buffer for SDS-PAGE (Powder)
Specification 10*500 mL
Introduction Hepes Running buffer is formulated for separation of proteins in their denaturing or nondenaturing state on Hepes-Tris gels. This product is available in powder form, a total of 10 packs, each pack can prepare 500 mL of 1× working solution.
Protocol (for reference only)
  • Take 1 pack of powder that can prepare 500 mL of hepes running buffer, pour it into a clean beaker, add 400 mL distilled water and stir to dissolve the powder completely.
  • Make up to 500 mL in the graduated cylinder with distilled water, which is 1× working solution and can be used after mixing.
  • Unused running buffer can be stored at room temperature, usually 2-3 days at room temperature and 1-2 weeks at 4°C.
Notes
  • 1× working buffer should not be used for more than 2 weeks after preparation.
  • This running buffer is suitable for Hepes-Tris gels, and should not be used for gels with other chemistries.
  • For research use only. Not for use in diagnostic procedures.
  • For your safety and health, please wear personal protective equipment and clothing when operating.
Storage Store at room temperature, valid for 2 years.

 

Features

  • Glass plate construction reduces non-specific protein adsorption, producing sharper and more defined protein bands.

  • Automated gel casting ensures batch stability and excellent reproducibility.

  • Wide 4–20% gradient provides strong resolving power across a broad molecular weight range.

  • Ideal for large molecular weight proteins — the gentle HEPES buffer system and gradient formulation help maintain separation quality of high-MW targets.

  • Optimized for membrane protein complexes — suitable for samples requiring mild conditions to preserve complex stability during electrophoresis.

  • SDS-free gel matrix allows both denaturing (SDS-PAGE) and non-denaturing (Native-PAGE) applications.

  • Ready-to-use format eliminates gel preparation time and reduces potential preparation errors.

  • Long shelf life and convenient storage at 2–8°C.

Applications

  • SDS-PAGE for general protein separation

  • Separation of large molecular weight proteins

  • Analysis of membrane protein complexes and other fragile protein assemblies

  • Native-PAGE for non-denaturing analysis

  • Western blot (WB) preparation and protein detection workflows

  • Any electrophoresis experiment requiring a 4–20% gradient gel

Storage

2~8°C, do not freeze

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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