Precast-Gel Hepes-Tris Native-PAGE 15% 10Well

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Sku: P310054-10 PCS
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Description

Precast-GLgel Hepes Native-PAGE 15% (10-Well) is a ready-to-use, non-denaturing gel designed for analyzing proteins in their native, biologically active conformations. The gel is constructed with an innovative glass plate design, which greatly reduces non-specific protein adsorption and ensures clearer and sharper native protein bands.

This 15% HEPES-based precast gel is supplied fully prepared with matched Native-PAGE running buffer, enabling direct sample loading without the need for manual gel formulation. The high acrylamide concentration offers excellent resolving power for small to medium molecular weight native proteins, and is also suitable for stabilizing compact membrane proteins and specific native protein complexes that benefit from a tighter gel matrix.

Specification

Full name Precast-GLgel Hepes Native-PAGE 15% 10-Well
Package 10 pcs
Components 10 pcs gel, 10 pcs Native-PAGE running buffer
Gel size 8 × 7.4 × 0.15 cm
Plate size 9.8 × 8.4 × 0.41 cm
Wells 10 wells
Buffer system HEPES buffer system
Maximum sample loading volume 60 µL
Recommended sample loading volume 30 µL

 

Buffers

Buffer Hepes Running Buffer for Native PAGE (Powder)
Specification 10*500 mL
Introduction Hepes Running buffer is formulated for separation of proteins in their denaturing or nondenaturing state on Hepes-Tris gels. This product is available in powder form, a total of 10 packs, each pack can prepare 500 mL of 1× working solution.
Protocol (for reference only)
  • Take 1 pack of powder that can prepare 500 mL of hepes running buffer, pour it into a clean beaker, add 400 mL distilled water and stir to dissolve the powder completely.
  • Make up to 500 mL in the graduated cylinder with distilled water, which is 1× working solution and can be used after mixing.
  • Unused running buffer can be stored at room temperature, usually 2-3 days at room temperature and 1-2 weeks at 4°C.
Notes
  • 1× working buffer should not be used for more than 2 weeks after preparation.
  • This running buffer is suitable for Hepes-Tris gels, and should not be used for gels with other chemistries.
  • For research use only. Not for use in diagnostic procedures.
  • For your safety and health, please wear personal protective equipment and clothing when operating.
Storage Store at room temperature, valid for 2 years.

 

Features

  • Glass plate construction effectively reduces non-specific protein adsorption, producing clearer and sharper native protein bands.

  • Automatic gel filling technology ensures consistent quality and excellent batch-to-batch reproducibility.

  • High-concentration 15% native gel provides strong resolving capacity for small to medium molecular weight native proteins.

  • Suitable for membrane proteins, especially compact or stable membrane-associated proteins that require a firm gel matrix.

  • Supports analysis of specific native protein complexes where high-percentage gels enhance band tightness and resolution.

  • SDS-free gel, ideal for non-denaturing electrophoresis and preserving protein structure and biological activity.

  • Ready-to-use precast format significantly reduces preparation time and eliminates manual gel-making steps.

  • Long shelf life, easily stored at 2–8°C.

Applications

  • Native-PAGE analysis of proteins in non-denatured form

  • Separation of small to medium molecular weight native proteins

  • Electrophoresis of membrane proteins

  • Analysis of native protein complexes that require high-percentage gels

  • Western blot (WB) after native separation

  • Experiments requiring a 15% native gel for compact protein resolution

Storage

2~8°C, do not freeze

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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