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Specification
| Full name | M-MuLV Reverse Transcriptase |
| Applications | First-strand cDNA synthesis for RT-PCR and real-time RT-PCR; synthesis of cDNA for cloning and expression studies; Preparation of labeled cDNA probes for microarray research; DNA marking; Primer extension method for RNA analysis |
| Summary | M-MuLV reverse transcriptase (RT) is a recombinant M-MuLV RT. It differs from M-MuLV RT in terms of structure and catalytic performance. The enzyme has RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity specific to RNA in RNA-DNA hybrids, which is significantly lower than that of avian myeloblastomavirus (AMV) reverse transcriptase RNase H Minus and acts in a wide range of temperatures (42~55°C). |
| Features | Efficient synthesis of full-length first-strand cDNA up to 13 kb, with activity up to 50°C at 42°C and addition of modified nucleotides |
| Composition | M-MuLV Reverse Transcriptase (200U/ul), 5X Reaction Buffer |
| Reaction Buffer | 5X Reaction Buffer |
| Unit Definition | One unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol dTMP into a polynucleotide fraction in 10 min at 37°C. |
| Thermal Inactivation | Heat at 70 °C for 10 min to lose activity. |
| Concentration | 200 U/μl |
| Storage Buffer | 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, 50% (v/v) glycerin. |
| Applications |
First-strand cDNA synthesis for RT-PCR and real-time RT-PCR; synthesis of cDNA for cloning and expression studies; Preparation of labeled cDNA probes for microarray research; DNA marking; Primer extension method for RNA analysis
|
| Component | M-MuLV Reverse Transcriptase (200U/ul), 5X Reaction Buffer |
Storage
-20°C
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
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