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Description
YM Medium is a specialized culture medium for Agrobacterium growth, transformation, and gene expression.
It is widely used in Agrobacterium electroporation and Agrobacterium-mediated plant transformation, where it significantly improves transformation efficiency compared with traditional LB broth.
Studies and internal validation show that YM Medium for Agrobacterium can increase electroporation efficiency by up to 40-fold, while effectively reducing bacterial cell aggregation. This results in more uniform cultures, making YM Medium an ideal Agrobacterium transformation medium for plant infection and downstream molecular biology applications.
Specification
| Appearance | Light beige, free-flowing powder |
| Conductivity (1:10) @25C | REPORT |
| pH (5.6g/ 500ml water)@25C | 6.2 ± 0.2 |
| Solubility (5.6g/ 500ml water) | PASS |
| Supports Growth of Bacteria | PASS |
| Component (g/L) | Yeast Extract 3.0g Peptone 5.0g D-glucose 10.0g Malt Extract 3.0g |
| Usage | 21.0g/L |
| Storage | Store at room temperature. Protect from moisture. |
Features
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Optimized Agrobacterium Culture Medium
Specially formulated as a culture medium for Agrobacterium, supporting robust growth, transformation, and gene expression.
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Enhanced Agrobacterium Transformation Efficiency
Significantly improves Agrobacterium electroporation efficiency compared to LB broth, enabling high-yield genetic transformation.
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Reduced Cell Aggregation During Culture
Promotes uniform bacterial suspension, which is critical for Agrobacterium-mediated plant transformation and infection studies.
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Rich and Balanced Nutrient Composition
Contains yeast extract, peptone, D-glucose, and malt extract to support vigorous and stable Agrobacterium growth.
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Easy Preparation and Stable Storage
Supplied as a free-flowing powder; suitable for routine laboratory use and long-term storage at room temperature.
Applications
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Cultivation of Agrobacterium species
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Agrobacterium-mediated plant transformation
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Agrobacterium electroporation and genetic engineering
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Plant molecular biology and functional genomics research
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Routine microbiological culture for plant infection studies
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
