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Description
YamayBio Western Blot Rapid Stripping buffers are essential tools for removing primary and secondary antibodies from membranes, enabling reprobing, saving time and costs when sample quantities are limited . YamayBio Western Blot Rapid Stripping Buffer is specifically formulated to gently and efficiently strip target proteins from PVDF and NC membranes, minimizing damage to the membrane. Its rapid action and odorless, environmentally friendly formulation ensure a safe and effective process.
Features
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Efficient:
Better than traditional stripping buffer.
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Mild and General:
Do not strip target.
The mild formula of Yamay Rapid Stripping buffer minimizes damage to antigens and membranes.
Fig 1. Hela cells lysate (2.5μg, 1.25μg, 0.62μg, 0.31μg of total protein) were probed with GAPDH. Following the initial detection,each blot was cut into two strips, one part of the blot was stripped in BM3154, the other was stripped in Thermo Scientific Restore Western Blot Stripping buffer (Part No.21059), for a long time stripping (6 hours). After the stripping procedure, the membrane strips were washed in 1× PBS Tween-20 buffer and incubated with the substrate and imaged.The membrane strips were reblocked and reprobed for GAPDH, the Western blot procedure repeated as described above.
The rapid stripping buffer is compatible with both PVDF and NC membranes.
Fig 2. A431 cells lysate (2.5μg, 1.25μg, 0.62μg, 0.31μg of total protein) were separated by SDS-PAGE and transferred to PVDF membranes or nitrocellulose (NC) membranes .The membranes were blocked by Protein-free Blocking Buffer (Part No.BM3152) and probed with an anti-GAPDH antibody. Following the initial detection, each blot was cut into two strips. One strip was stripped in Yamay rapid stripping buffer BM3154, while the other was stripped in Thermo Scientific Restore Western Blot Stripping buffer (Part No.21059) for 15 minutes at room temperature, according to the manufacturer's instructions. After the stripping procedure, the membrane strips were washed in 1× PBS Tween-20 buffer and incubated with the substrate and imaged. The membrane strips were reblocked and reprobed for an anti-HSP60 antibody.
Specifications
| Product Type | Western Blot Stripping Buffer |
| Form | Liquid |
| Application | Western Blot |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Quantity | 100 mL / 500 mL |
Storage
Transported at room temperature and stored at 4°C. The shelf life is 12 months.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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