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$61.00
Sku: CB0354-100mg
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by July 14, 2026
Description
In many cell biology and mitochondrial research workflows, inconsistent detection of apoptosis signals and variable mitochondrial respiration readouts often arise from unstable or low-quality reference proteins. These issues can lead to unreliable interpretation of oxidative phosphorylation efficiency, electron transport chain activity, and caspase activation events.
Cytochrome C (CB0354) is a highly purified mitochondrial heme protein widely used as a reference standard for studying the mitochondrial electron transport chain and intrinsic apoptosis pathway. As a soluble protein located in the intermembrane space of mitochondria, Cytochrome C plays a central role in transferring electrons between Complex III and Complex IV, supporting efficient oxidative phosphorylation and ATP generation.
Beyond its metabolic function, Cytochrome C is a critical apoptosis marker protein. Upon mitochondrial outer membrane permeabilization, it is released into the cytosol and triggers caspase cascade activation, making it an essential tool for cell death mechanism research, mitochondrial dysfunction studies, and redox biology assays.
Specification
| Product Name | Cytochrome C |
| Catalog No. | CB0354 |
| CAS No. | 9007-43-6 |
| Molecular Weight | ~12,588 Da |
| Source | Porcine heart |
| Appearance | Crystalline solid (reddish-brown) |
| Purity | ≥95% (HPLC) |
| Loss on Drying | ≤5.0% |
| Solubility | 100 g/L (20°C, water) |
| Storage | -20°C |
| Bio Safety | Non-hazardous (RUO) |
Features
- Highly purified mitochondrial Cytochrome C protein for reproducible experimental performance
- Validated apoptosis marker for caspase activation and intrinsic pathway studies
- Key reference standard for mitochondrial electron transport chain assays
- Excellent solubility for biochemical and cell-based applications
- Chromatography purified with consistent lot-to-lot stability
- Suitable for redox reaction and oxidative phosphorylation research models
Applications
- Mitochondrial electron transport chain and respiration studies
- Apoptosis assay and caspase activation pathway analysis
- Cell death mechanism and intrinsic apoptosis research
- Oxidative phosphorylation and ATP production studies
- Western blot and apoptosis marker reference protein
- Redox reaction and enzymology model system
- Mitochondrial dysfunction and oxidative stress research
Biological Function Overview
Cytochrome C functions as a central electron carrier in the mitochondrial respiratory chain, transferring electrons from Complex III to Complex IV. This process is essential for maintaining efficient oxidative phosphorylation and cellular ATP production.
During cellular stress or damage, Cytochrome C is released into the cytoplasm, where it initiates apoptosome formation and activates caspase enzymes. This makes it a key molecular switch between cell survival and programmed cell death.
Storage & Handling
- Store at -20°C for long-term stability
- Avoid repeated freeze-thaw cycles
- Keep sealed and protected from moisture and light
Documents
Frequently Asked Questions
What is Cytochrome C used for in research?
Cytochrome C is widely used as a mitochondrial electron transport chain protein and apoptosis marker for studying cell death, caspase activation, and oxidative phosphorylation.
How does Cytochrome C function in apoptosis?
It is released from mitochondria into the cytosol, where it activates apoptosome formation and triggers caspase-dependent programmed cell death.
Can Cytochrome C be used as a Western blot marker?
Yes, it is commonly used as an apoptosis marker protein in Western blot and mitochondrial dysfunction studies.
What purity level is recommended?
≥95% purity (HPLC) is recommended for reliable biochemical and cell-based experiments.
How should Cytochrome C be stored?
Store at -20°C and avoid repeated freeze-thaw cycles to maintain protein stability.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
