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Ready-to-Use Bradford Reagent for Rapid Protein Quantification at 595 nm
$23.00
Sku: BDE641-100mL
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by July 29, 2026
Description
Reliable protein concentration measurement is an important step in routine biochemical workflows. Laboratories require a protein assay that is rapid, economical, easy to perform, and compatible with commonly available spectrophotometers or microplate readers.
Bradford Reagent is a ready-to-use colorimetric protein assay reagent based on the binding of Coomassie Brilliant Blue G-250 to protein. Formation of the dye-protein complex produces an absorbance response that is measured spectrophotometrically at 595 nm, allowing the concentration of an unknown protein sample to be calculated from a protein standard curve.
The reagent provides a documented linear relationship across a protein concentration range of 10–150 µg/mL and can detect a minimum protein quantity of 0.2 µg in a 1–20 µL sample volume. The assay procedure for approximately 10–20 samples can be completed in about 10 minutes, excluding sample preparation time.
Separate protocols are provided for test-tube measurements and 96-well plate protein assays, supporting both conventional spectrophotometric analysis and microplate-based protein quantification.
Assay Highlights
595 nm
Colorimetric absorbance measurement
10–150 µg/mL
Documented linear protein range
0.2 µg
Minimum detectable protein quantity
10 Minutes
Room-temperature incubation
Specification
| Product Name | Bradford Reagent |
| Product Code | BDE641 |
| Assay Type | Colorimetric protein assay |
| Assay Principle | Coomassie Brilliant Blue G-250 dye binding |
| Detection Wavelength | 595 nm |
| Linear Range | 10–150 µg/mL protein |
| Recommended Protocol Range | 10–125 µg/mL |
| Minimum Protein Quantity | 0.2 µg in a 1–20 µL sample volume |
| Assay Formats | Test tube and 96-well plate |
| Incubation | 10 minutes at room temperature |
| Appearance | Clear blue liquid |
| Storage | 2–8°C |
| Intended Use | For laboratory research use only |
Features
- Rapid protein quantification: The assay workflow for approximately 10–20 samples can be completed in about 10 minutes, excluding sample preparation.
- High-sensitivity protein detection: Detects a minimum protein quantity of 0.2 µg in a 1–20 µL sample volume.
- Defined linear response: Provides a documented linear relationship across a 10–150 µg/mL protein concentration range.
- Ready-to-use Bradford reagent: Eliminates the need to prepare the Coomassie Brilliant Blue G-250 assay reagent before routine measurements.
- Two assay formats: Includes procedures for both test-tube protein assays and 96-well microplate assays.
- Standard laboratory detection: Compatible with absorbance measurement at 595 nm using a spectrophotometer or microplate reader.
- Economical workflow: Designed for direct measurement of total protein concentration with a straightforward dye-binding assay procedure.
Applications
Bradford Reagent is intended for direct colorimetric determination of total protein concentration. The assay is suitable for research laboratories performing routine protein quantification from unknown samples using a BSA standard curve or a standard prepared from the protein of interest.
- Total protein quantification: Determine the concentration of unknown protein samples through absorbance measurement at 595 nm.
- Bradford protein assay standard curves: Generate blank-corrected calibration curves using BSA protein standards.
- Test-tube protein assays: Perform conventional protein concentration measurements using a spectrophotometer and cuvettes.
- 96-well plate protein assays: Process multiple standards and unknown samples using a microplate reader.
- Routine protein concentration measurement: Support day-to-day protein analysis in biochemical and molecular research laboratories.
- Comparative protein measurements: Evaluate unknown samples prepared at different dilution factors within the recommended assay range.
- Protein-specific calibration: Improve measurement relevance by preparing a standard curve with the protein of interest when appropriate.
Assay Formats
Test-Tube Bradford Assay
- 100 µL standard or diluted sample
- 1 mL Bradford Reagent
- Mix thoroughly
- Incubate for 10 minutes at room temperature
- Measure absorbance at 595 nm
96-Well Plate Bradford Assay
- 20 µL standard or diluted sample
- 200 µL Bradford Reagent
- Mix on a plate shaker for 30 seconds
- Incubate for 10 minutes at room temperature
- Measure absorbance at 595 nm
Bradford Protein Assay Workflow
1. Prepare
Prepare protein standards and diluted unknown samples.
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2. Add Reagent
Add Bradford Reagent to each standard, blank and unknown.
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3. Incubate
Mix and incubate for 10 minutes at room temperature.
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4. Read
Measure absorbance at 595 nm.
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5. Calculate
Use the standard curve and dilution factor to calculate protein concentration.
Quality Control
| Test Item | Specification |
|---|---|
| Appearance | Clear blue liquid |
| Absorbance at 595 nm | >1.80 |
| Characteristic Pattern | Pass |
| Peak A | 463–467 nm |
Lot-specific results should be confirmed using the Certificate of Analysis supplied for the applicable production lot.
Important Assay Notes
- Mix the Bradford Reagent thoroughly before use.
- Allow the reagent to warm to room temperature before use.
- Prepare and measure standards and unknown samples in duplicate to reduce experimental error.
- Generate a new protein standard curve for each assay.
- Measure samples from the lowest protein concentration to the highest concentration.
- For greater relevance, use the protein of interest to prepare the standard curve when appropriate.
- For samples containing detergent, the product instructions recommend using a BCA protein assay rather than this Bradford assay.
Storage and Handling
Store the Bradford Reagent tightly closed at 2–8°C. Keep the product in its original packaging and allow it to warm to room temperature before opening and use.
The reagent contains an acidic Coomassie Brilliant Blue G-250 formulation. Wear suitable protective gloves and eye protection, avoid skin and eye contact, and handle the product according to the Safety Data Sheet and established laboratory safety procedures.
Documents
FAQ
What is Bradford Reagent used for?
Bradford Reagent is used for direct colorimetric determination of total protein concentration. Unknown samples are measured against a protein standard curve, and absorbance is read at 595 nm.
How does the Bradford protein assay work?
The assay uses Coomassie Brilliant Blue G-250 dye. When the dye binds to protein, a dye-protein complex forms and produces an absorbance response that can be measured spectrophotometrically at 595 nm.
What is the linear range of this Bradford protein assay?
The product information documents a good linear relationship across a protein concentration range of 10–150 µg/mL. The supplied protocols prepare standards from 10–125 µg/mL.
What is the minimum amount of protein that can be detected?
The documented minimum detectable protein quantity is 0.2 µg in a sample volume of 1–20 µL.
At what wavelength should Bradford assay absorbance be measured?
Measure the absorbance of standards, blanks and unknown protein samples at 595 nm.
Can this Bradford Reagent be used in a 96-well plate?
Yes. The supplied 96-well plate protocol uses 20 µL of standard or diluted protein sample and 200 µL of Bradford Reagent per well. After mixing for 30 seconds and incubating for 10 minutes at room temperature, absorbance is measured at 595 nm.
Can the reagent be used for test-tube protein assays?
Yes. The test-tube protocol uses 100 µL of standard or diluted protein sample with 1 mL of Bradford Reagent. Samples are mixed, incubated for 10 minutes at room temperature and measured at 595 nm.
How long should samples be incubated with Bradford Reagent?
Incubate standards and unknown protein samples with the reagent for 10 minutes at room temperature before measuring absorbance.
Should Bradford Reagent be warmed before use?
Yes. Warm the reagent to room temperature and mix it thoroughly before beginning the protein assay.
What protein standard should be used for the standard curve?
The supplied protocol uses BSA standards. Because absorbance response can vary between proteins, the instructions also state that results may be more accurate when the protein of interest is used to prepare the standard curve.
Can detergent-containing samples be measured with this Bradford assay?
The product instructions recommend using a BCA Protein Assay Kit when detergent is present in the sample. Detergent-containing samples should therefore be evaluated with the recommended alternative assay.
Should standards and unknown samples be measured in duplicate?
Yes. Duplicate measurements are recommended as good laboratory practice to reduce error. A new standard curve should be generated for each assay.
How is protein concentration calculated from the Bradford assay?
Subtract the average blank absorbance from the standards and unknown samples, plot the blank-corrected protein standard curve, determine the concentration of the diluted sample and then apply the recorded dilution factor to calculate the original protein concentration.
How should Bradford Reagent be stored?
Store the reagent tightly closed at 2–8°C. Allow it to warm to room temperature before opening and use.
Where can I find the protocol, COA and Safety Data Sheet?
The product protocol, Certificate of Analysis and Safety Data Sheet are available in the Documents section of this product page.
For laboratory research use only. Not for human or animal drug use.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
