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$108.00
Sku: BF-T1008-V2-S
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by October 13, 2026
Description
The Biofargo ssDNA Assay Kit-V2 provides fluorescence-based quantification of single-stranded DNA (ssDNA) for molecular biology research. The reagent also responds to dsDNA and RNA; purify ssDNA before quantification when these are present.
The total target amount is 1–200 ng per assay. Use 1–20 µL of sample in a 200 µL final reaction and incubate for 2 minutes at 20–25 °C, protected from light. Prepare fresh working solution by mixing 1 volume of fluorescent reagent with 199 volumes of the supplied buffer (1:200 final dilution).
Available formats: BF-T1008-V2-S (100 assays) and BF-T1008-V2 (1,000 assays). For research use only. Not for diagnostic or therapeutic procedures. This assay does not independently distinguish ssDNA from contaminating dsDNA or RNA.
Specifications
| Parameter | Specification |
| Biofargo catalog numbers | BF-T1008-V2-S / BF-T1008-V2 |
| Target | single-stranded DNA (ssDNA) |
| Total target amount per assay | 1–200 ng |
| Initial sample concentration | 0.05–200 ng/µL (50 pg/µL at the lower end), depending on sample input |
| Sample input | 1–20 µL |
| Final assay volume | 200 µL per tube or well |
| Working-solution preparation | Prepare fresh working solution by mixing 1 volume of fluorescent reagent with 199 volumes of the supplied buffer (1:200 final dilution). |
| Incubation | 2 minutes at 20–25 °C, protected from light |
| Signal stability | Up to 3 hours, protected from light |
| Instrument formats | Biofargo X1 and X3; Qubit fluorometers with the matching assay; fluorescence microplate readers |
| Plate-reader settings | 485 nm excitation / 530 nm emission |
| Available formats | BF-T1008-V2-S: 100 assays; BF-T1008-V2: 1,000 assays |
| Intended use | Research use only |
Features
Concentrated fluorescent reagent and assay buffer support freshly prepared working solution.
Quantifies single-stranded DNA (ssDNA) at 1–200 ng per assay.
The reagent also responds to dsDNA and RNA; purify ssDNA before quantification when these are present.
Uses 1–20 µL of sample in a 200 µL reaction.
Two-minute incubation at room temperature.
Supports tube-based and fluorescence microplate-reader workflows.
Applications
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Oligonucleotide and ssDNA research: Quantify purified oligonucleotides or long ssDNA. Sequence, length and contamination can affect the signal.
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NGS sample preparation: Measure target nucleic acid concentration for input normalization; this concentration assay does not measure fragment size, integrity or amplifiable library concentration.
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Fluorescence microplates: Use a black 96-well plate and a multi-point standard curve at 485 nm excitation / 530 nm emission.
Kit Components
| Component | Designation | BF-T1008-V2-S (100 assays) | BF-T1008-V2 (1,000 assays) | Function |
| ssDNA Buffer QB-V2 | QB-V2 | 20 mL | 200 mL | Working-solution buffer |
| ssDNA Standard QS0 | QS0 | 100 µL | 1 mL | Blank standard; 0 ng/µL |
| ssDNA Standard QS1 | QS1 | 100 µL | 1 mL | High standard; 20 ng/µL |
| ssDNA Fluorescent Reagent QF | QF | 100 µL | 1 mL | Concentrated detection reagent |
Storage & Shipping
Ship on ice.
Store all components at 2–8 °C, protected from light.
Frequently Asked Questions (FAQs)
What is the quantification range?
The target mass range is 1–200 ng in the assay. The stated initial concentration range is 0.05–200 ng/µL (50 pg/µL at the lower end), depending on sample input. Initial sample concentration is measured before dilution; target mass equals initial concentration multiplied by the sample volume. These are different quantities.
Can dsDNA or RNA affect ssDNA results?
Yes. The ssDNA reagent also produces fluorescence with dsDNA and RNA. Remove these contaminants before interpreting a result as ssDNA concentration.
How do I prepare the working solution?
Prepare fresh working solution by mixing 1 volume of fluorescent reagent with 199 volumes of the supplied buffer (1:200 final dilution).
Can I use this kit with Qubit fluorometers?
The supplier protocol supports use with the matching Qubit assay program. Use this kit's own standards, the specified sample volume and the correct consumables for the Qubit model. Compatibility does not imply Thermo Fisher endorsement or identical performance. For the 1X assay on older Qubit models, verify that the appropriate assay program is installed.
Which Biofargo fluorometers support this assay?
X1 and X3 list the corresponding dsDNA or ssDNA program. The eight-position X3 uses different tubes from the single-sample X1.
How much sample is required?
Add 1–20 µL of sample and adjust the working solution to a final volume of 200 µL. Choose the input volume so the target mass lies within the assay range. Dilute samples that exceed the upper limit.
Which assay tubes should I use?
Use clear, thin-wall 0.5 mL assay tubes for Biofargo X1 and Qubit 2/3/4. Use the instrument-specified clear 0.2 mL tubes or eight-tube strips for Biofargo X3; Qubit Flex requires its specified 0.2 mL assay tube strips. Keep the total assay volume at 200 µL.
How long does the assay take?
Incubate for 2 minutes at 20–25 °C, protected from light, after mixing. This is the incubation time, not the total preparation and measurement time. The signal remains stable for up to 3 hours when protected from light.
Do I need new standards?
Use the two standards supplied with this kit and the matching assay program. A new calibration for each run is recommended. Do not reuse a calibration generated with a different kit. For a plate reader, prepare a dilution series and fit a standard curve.
Does the reaction count equal the number of unknown samples?
The listed count is the nominal assay format. Standards, controls, repeat measurements and handling losses reduce the number of unknown samples that can be measured.
How should I store the kit?
Ship on ice. Store all components at 2–8 °C, protected from light.
Is this product intended for diagnostic use?
No. For research use only. Not for diagnostic or therapeutic procedures.
Compatible Instruments
This kit is run on the Biofargo fluorometers below.
Biofargo Fluorometer X1 — Single sample position. Select the matching assay program on the instrument.
Biofargo Fluorometer X3 — Eight sample positions. Select the matching assay program on the instrument.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
