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$369.00
Sku: BFMAT10
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by November 04, 2026
Description
Color interference, undefined growth factor activity and inconsistent gel formation can complicate organoid and 3D cell culture. These variables make it harder to separate true experimental responses from signals introduced by the matrix itself, especially in fluorescence assays and studies that rely on controlled growth factor supplementation.
Biofargo Growth Factor Reduced, Phenol Red-Free Basement Membrane Matrix (BFMAT10) is a natural basement membrane extract from mouse tumor tissue. It provides a laminin-rich 3D matrix containing collagen IV, entactin and heparan sulfate proteoglycans for cell embedding, matrix coating and organoid culture.
The matrix is supplied at 8–12 mg/mL in high-glucose, phenol red-free DMEM. It stays liquid at 4°C for handling and dispensing, and forms a stable hydrogel on warming, with gelation fastest at 37°C. Every batch is evaluated in an organoid culture test and screened for mycoplasma, bacteria, fungi and LDEV.
Specifications
| Product Name | Biofargo Growth Factor Reduced, Phenol Red-Free Basement Membrane Matrix |
| SKU | BFMAT10 |
| Product Type | Growth factor reduced, phenol red-free basement membrane matrix |
| Matrix Source | Mouse tumor-derived basement membrane extract |
| Size | 10 mL |
| Protein Concentration | 8–12 mg/mL (BCA) |
| Appearance | Translucent, pale yellow |
| Physical State | Liquid after thawing at 4°C |
| Diluent | High-glucose, phenol red-free DMEM |
| Antibiotic | 50 μg/mL gentamicin (standard, non-custom formulations) |
| Endotoxin | ≤10 EU/mL |
| Gelation Time | About 5–30 minutes at room temperature; faster at 37°C |
| Storage | ≤−20°C |
| Shipping Condition | Frozen, ≤−20°C |
| Shelf Life | 24 months |
| Grade | For research use only |
Matrix Composition
Laminin — major basement membrane glycoprotein involved in cell adhesion and matrix organization
Collagen IV — structural collagen forming the basement membrane network
Entactin (nidogen) — links the laminin and collagen IV networks
Heparan sulfate proteoglycans — contribute to matrix structure and molecular interactions
As a natural tumor-derived extracellular matrix, the product may contain residual endogenous factors such as IGF-1, TGF-β, VEGF, EGF and bFGF. The growth factor reduced formulation lowers, but does not completely remove, this background activity.
Features
Growth factor reduced — lower endogenous signaling background for growth factor-controlled experiments
Phenol red-free — minimizes color and fluorescence interference in imaging
Native basement membrane proteins — laminin, collagen IV, entactin and HSPGs
Consistent concentration — protein controlled within 8–12 mg/mL
Temperature-responsive gelation — easy cold handling, rapid gel formation on warming
Organoid-tested — every batch evaluated in an organoid culture test
Safety screened — tested for mycoplasma, bacteria and fungi; broad PCR pathogen panel including LDEV
Quality Control
| Quality Attribute | Test or Acceptance Criterion |
|---|---|
| Mycoplasma | Not detected |
| Bacterial Contamination | Not detected |
| Fungal Contamination | Not detected |
| Endotoxin | Gel-clot limit test; specification ≤10 EU/mL |
| Protein Concentration | BCA assay |
| Pathogen Screening | Broad PCR-based panel, including LDEV |
| Functional Evaluation | Organoid culture test on every batch |
Gelation and Stability
The thawed matrix stays liquid at 4°C and gels as temperature rises: about 5–30 minutes at room temperature, faster at 37°C.
Evaluated 1:2 Dilution
Diluted 1:2 with culture medium and incubated at 37°C for 30 minutes, the matrix formed a gel that kept its structure for 5 days at 37°C after medium was added.
Evaluated 70% Matrix Condition
At 70% final matrix content, dispensed at 50 μL per well and incubated at 37°C for 30 minutes, the matrix formed a stable gel that kept its structure for up to 15 days at 37°C after medium was added.
Important: These are reference conditions, not a universal protocol. Optimize matrix concentration, volume and gelation time for each cell type, vessel and workflow.
Applications
Organoid Culture and Expansion
Organoid establishment, embedding and 3D expansion (human or animal)
Long-term organoid culture requiring a stable matrix
Organoid morphology and imaging studies
3D Cell Culture and Matrix Coating
3D cell embedding and basement membrane coating
Cell–matrix interaction and ECM-dependent morphology research
Growth Factor-Controlled Research
Studies with controlled addition of exogenous growth factors or inhibitors
Signaling research requiring reduced matrix-derived background
Fluorescence and Live-Cell Imaging
Fluorescence, confocal and live-cell imaging of embedded cells or organoids
Image-based analysis where phenol red is undesirable
Cancer and Tumor Models
Tumor and patient-derived organoid culture
Drug-response studies using matrix-embedded organoids
Recommended Handling
Store unopened product at or below −20°C.
Thaw at about 4°C (refrigerator or on ice).
Pre-chill pipette tips, tubes and culture vessels to prevent premature gelation.
Mix gently while cold; avoid introducing bubbles.
Dispense while liquid, then incubate as required by your protocol.
Formulation note: Standard non-custom products contain 50 μg/mL gentamicin. Take this into account for antibiotic-free experiments.
Complete Your Organoid Workflow
Organoid media: Mouse Intestinal, Mouse Liver, Mouse Lung, Human Colon Cancer and Human Lung Cancer Organoid Medium Kits
Tissue processing: Organoid Tissue Digestion Solution and Intestinal Organoid Digestion Solution
Banking: OrgCryo Protein-Free Organoid Cryopreservation Solution
iPSC work: Basement Membrane Matrix, iPSC-Qualified
FAQ
What is BFMAT10 used for?
Organoid culture, 3D cell culture, matrix coating, cell embedding and fluorescence imaging studies.
How is growth factor reduced matrix different from standard basement membrane matrix?
It contains lower levels of endogenous growth factors, reducing matrix-derived background when you study defined growth factor conditions. It is not completely growth factor-free.
Why choose a phenol red-free matrix?
It minimizes color and optical background, which helps fluorescence, confocal and live-cell imaging.
Is BFMAT10 suitable for organoid culture?
Yes. Every batch is evaluated in an organoid culture test. Optimize concentration, dome volume and protocol for your organoid type.
What is the protein concentration?
8–12 mg/mL, measured by BCA.
How fast does it gel?
About 5–30 minutes at room temperature, faster at 37°C. Actual time depends on concentration, volume and handling temperature.
Can it be diluted with culture medium?
Yes. Evaluated conditions include 1:2 dilution (gel stable 5 days) and 70% matrix (gel stable up to 15 days). Dilution affects gel strength, so optimize for your application.
Does it contain antibiotics?
Standard formulations contain 50 μg/mL gentamicin.
What contamination testing is performed?
Mycoplasma, bacteria and fungi testing, plus a broad PCR pathogen panel including LDEV. Endotoxin specification is ≤10 EU/mL.
How should it be stored and thawed?
Store at or below −20°C (24-month shelf life). Thaw at about 4°C and keep cold during preparation.
Is it for clinical use?
No. For research use only.
For Research Use Only. Not for use in diagnostic procedures.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
